Click image to see more details
Product Info Summary
| SKU: | PB9681 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB, ELISA (Cap) |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Lymphotactin/XCL1 Antibody Picoband®
SKU/Catalog Number
PB9681
PB0702 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Lymphotactin/XCL1 Antibody Picoband® catalog # PB9681. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lymphotactin/XCL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9681)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Lymphotactin recombinant protein (Position: V22-G114). Human Lymphotactin shares 60.2% and 57% amino acid (aa) sequence identity with mouse and rat Lymphotactin, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9681 is reactive to XCL1 in Human
Observed Molecular Weight
13 kDa
Calculated molecular weight
12.5 kDa
Background of XCL1
Chemokine (C motif) ligand (XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. Muller et al. (1995) mapped the gene to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9681 is guaranteed for WB, ELISA (Cap) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
ELISA (Cap), 1-5μg/ml
Positive Control
WB: HELA Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Lymphotactin using anti-Lymphotactin antibody (PB9681).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HELA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lymphotactin antigen affinity purified polyclonal antibody (Catalog # PB9681) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Lymphotactin at approximately 13 kDa. The expected band size for Lymphotactin is at 13 kDa.
Click image to see more details
Sandwich ELISA - Recombinant human Lymphotactin/XCL1 protein standard curve.
Use in combination with reagents from Human Lymphotactin/XCL1 ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ0802).
Specific Publications For Anti-Lymphotactin/XCL1 Antibody Picoband® (PB9681)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Lymphotactin/XCL1 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Lymphotactin/XCL1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Lymphotactin/XCL1 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood using anti-Lymphotactin/XCL1 antibody PB9681. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-30
Question
Is a blocking peptide available for product anti-Lymphotactin/XCL1 antibody (PB9681)?
Verified Customer
Verified customer
Asked: 2019-01-15
Answer
We do provide the blocking peptide for product anti-Lymphotactin/XCL1 antibody (PB9681). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-01-15
Question
Does PB9681 anti-Lymphotactin/XCL1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Zhang
Verified customer
Asked: 2018-06-20
Answer
You can see on the product datasheet, PB9681 anti-Lymphotactin/XCL1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-20
Question
We are currently using anti-Lymphotactin/XCL1 antibody PB9681 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-16
Answer
The anti-Lymphotactin/XCL1 antibody (PB9681) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-16
Question
See attached the WB image, lot number and protocol we used for peripheral blood using anti-Lymphotactin/XCL1 antibody PB9681. Please let me know if you require anything else.
M. Zhang
Verified customer
Asked: 2015-11-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-11-16
