Anti-XIAP Antibody Picoband™ (monoclonal, 3G2G1)

Product Name

Anti-XIAP Antibody Picoband™ (monoclonal, 3G2G1)

View all XIAP Antibodies

SKU/Catalog Number

M00482-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-XIAP Antibody Picoband™ (monoclonal, 3G2G1) catalog # M00482-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-XIAP Antibody Picoband™ (monoclonal, 3G2G1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00482-1)

Host

Mouse

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Monoclonal

Clone Number

3G2G1

Isotype

Mouse IgG1

Immunogen

E.coli-derived human XIAP recombinant protein (Position: A15-V244). Human XIAP shares 89.4% and 90.7% amino acid (aa) sequence identity with mouse and rat XIAP, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00482-1 is reactive to XIAP in Human, Mouse, Rat

Applications

M00482-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

56.685kDa

Background of XIAP

XIAP, also known as IAP3 or BIRC4, is a protein that stops apoptotic cell death. It is mapped to chromosome Xq25. This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of XIAP using anti-XIAP antibody (M00482-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human PANC-1 whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-XIAP antigen affinity purified monoclonal antibody (Catalog # M00482-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for XIAP at approximately 54 kDa. The expected band size for XIAP is at 57 kDa.

Gene/Protein Information For XIAP (Source: Uniprot.org, NCBI)

Alternative Names

API3X-linked inhibitor of apoptosis protein; apoptosis inhibitor 3; baculoviral IAP repeat-containing 4; baculoviral IAP repeat-containing protein 4; BIRC4; BIRC4XLP2; E3 ubiquitin-protein ligase XIAP; EC 6.3.2.-; hIAP3; hIAP-3; hILP; IAP3; IAP-3; IAP-like protein; ILP; ILP1; Inhibitor of apoptosis protein 3; MIHA; XIAP; X-linked IAP; X-linked inhibitor of apoptosis XIAP API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3 X-linked inhibitor of apoptosis E3 ubiquitin-protein ligase XIAP|IAP-like protein 1|RING-type E3 ubiquitin transferase XIAP|X-linked IAP|X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase|baculoviral IAP repeat-containing protein 4|inhibitor of apoptosis protein 3

For more info on XIAP, check out the XIAP Infographic

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In this infographic, you will see the following information for XIAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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