Anti-Phospho-XIRP1 (Ser295) Antibody

Product Name

Anti-Phospho-XIRP1 (Ser295) Antibody

View all Xirp1 Antibodies

SKU/Catalog Number

P07785

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-XIRP1 (Ser295) Antibody (Catalog # P07785). Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-XIRP1 (Ser295) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P07785)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 of mouse XIRP, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Mouse.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P07785 is reactive to Xirp1 in Mouse

Applications

P07785 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

123.431kDa

Background of Xirp1

XIRP1 is a member of the Xin family of proteins, containing 16 Xin repeats. The intercalated disk protein Xin protects actin filaments from depolymerization, and is able to bundle actin filaments to interact with β-catenin, which is thought to stabilize actin-based cytoskeletons (Choi et al., 2007). The evolutionary emergence of the Xin paralogs may have played a key role in the development of heart chambers with complete endothelial and myocardial layers (Grosskurth et al., 2008). Loss of mXina (mouse Xin homolog) results in cardiac hypertrophy and cardiomyopathy (Gustafson-Wagner et al., 2007). Phosphorylation of Ser-295 plays a key role in XIRP1 signaling, and therefore in cardiac hypertrophy, as it has been shown that acute pressure-overloaded hearts rapidly increased phosphorylation at the Ser-295 residue of XIRP1. (Chang et al., 2013).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of mouse heart lysate showing specific immunolabeling of the ~250 kDa and ~150 kDa splice variants of the XIRP1 protein phosphorylated at Ser²⁹⁵ in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 minutes).

Gene/Protein Information For Xirp1 (Source: Uniprot.org, NCBI)

Alternative Names

cardiomyopathy associated 1; CMYA1; DKFZp779C1255; xin actin-binding repeat containing 1 Xirp1|AI415219, Cmya, Cmya1, X|xin actin-binding repeat containing 1|xin actin-binding repeat-containing protein 1|Xin alpha|cardiomyopathy associated 1|cardiomyopathy-associated protein 1|mXin alpha

For more info on Xirp1, check out the Xirp1 Infographic

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In this infographic, you will see the following information for Xirp1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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