Anti-XTP3TPA/DCTPP1 Antibody Picoband™

Product Name

Anti-XTP3TPA/DCTPP1 Antibody Picoband™

View all XTP3TPA Antibodies

SKU/Catalog Number

A11334-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-XTP3TPA/DCTPP1 Antibody Picoband™ catalog # A11334-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-XTP3TPA/DCTPP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11334-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human XTP3TPA/DCTPP1 recombinant protein (Position: R22-T170).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A11334-1 is reactive to Dctpp1 in Human, Mouse

Applications

A11334-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

19 kDa

Calculated molecular weight

18.795kDa

Background of XTP3TPA

dCTP pyrophosphatase 1 is an enzyme that in humans is encoded by the DCTPP1 gene. The protein encoded by this gene is dCTP pyrophosphatase, which converts dCTP to dCMP and inorganic pyrophosphate. The encoded protein also displays weak activity against dTTP and dATP, but none against dGTP. This protein may be responsible for eliminating excess dCTP after DNA synthesis and may prevent overmethylation of CpG islands. Three transcript variants, one protein-coding and the other two non-protein coding, have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of XTP3TPA/DCTPP1 using anti-XTP3TPA/DCTPP1 antibody (A11334-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-XTP3TPA/DCTPP1 antigen affinity purified polyclonal antibody (Catalog # A11334-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for XTP3TPA/DCTPP1 at approximately 19 kDa. The expected band size for XTP3TPA/DCTPP1 is at 19 kDa.

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Figure 2. Flow Cytometry analysis of JK cells using anti-XTP3TPA/DCTPP1 antibody (A11334-1).
Overlay histogram showing JK cells stained with A11334-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-XTP3TPA/DCTPP1 Antibody (A11334-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For Dctpp1 (Source: Uniprot.org, NCBI)

Alternative Names

dCTP pyrophosphatase 1; dCTPase 1; Deoxycytidine-triphosphatase 1; EC 3.6.1.12; MGC5627; RS21C6XTP3-transactivated gene A protein; XTP3TPACDA03; XTP3-transactivated protein A

For more info on Dctpp1, check out the Dctpp1 Infographic

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In this infographic, you will see the following information for Dctpp1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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