Anti-Y-box-binding protein 2 YBX2 Antibody

Product Name

Anti-Y-box-binding protein 2 YBX2 Antibody

View all YBX2 Antibodies

SKU/Catalog Number

A08610

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Y-box-binding protein 2 YBX2 Antibody (Catalog # A08610). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

YBX2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Y-box-binding protein 2 YBX2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08610)

Host

Rabbit

Contents

YBX2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

YBX2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy end of human YBX2. The immunogen is located within amino acids 300 - 350 of YBX2.

Reactive Species

A08610 is reactive to YBX2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

38.5 kDa

Background of YBX2

Germ cell specific Y-box binding protein (YBX2), a germ-cell-specific member of the Y-box family of DNA-/RNA-binding proteins, is a major constituent of messenger ribonucleoprotein particles (mRNPs) and involved in the regulation of the stability and/or translation of germ cell mRNAs. It is proposed to function as a coactivator of transcription in the nucleus and to stabilize and store maternal and paternal mRNAs in the cytoplasm. YBX2 binds to the Y-box consensus promoter element and is expressed in oocytes and testicular germ cells in the stage of spermatogonia to spermatocyte. Recent studies show that deletion of YBX2 leads to the disruption of both spermatogenesis and oogenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08610 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

YBX2 antibody can be used for detection of YBX2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information YBX2

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