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Product Info Summary
| SKU: | A01054-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-YB1/Ybx1 Antibody Picoband®
SKU/Catalog Number
A01054-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-YB1/Ybx1 Antibody Picoband® catalog # A01054-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-YB1/Ybx1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01054-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse YB1/Ybx1, identical to the related rat sequences.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01054-1 is reactive to Ybx1 in Mouse, Rat
Observed Molecular Weight
50 kDa
Calculated molecular weight
35.7 kDa
Background of Ybx1
Y box binding protein 1 also known as Y-box transcription factor or nuclease-sensitive element-binding protein 1 is a protein that in humans is encoded by the YBX1 gene. Enables chromatin binding activity; mRNA binding activity; and single-stranded DNA binding activity. Involved in epidermis development and negative regulation of cellular senescence. Acts upstream of or within several processes, including cellular response to interleukin-7; negative regulation of striated muscle cell differentiation; and negative regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus. Part of histone pre-mRNA 3'end processing complex. Is expressed in several structures, including branchial arch; central nervous system; facial prominence; gonad; and retina. Orthologous to human YBX1 (Y-box binding protein 1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01054-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Mouse
Positive Control
WB: rat RH-35 whole cell, mouse spleen tissue, mouse testis tissue, mouse Neuro-2a whole cell
FCM: ANA-1 cell
Validation Images & Assay Conditions
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Western blot analysis of YB1/Ybx1 using anti-YB1/Ybx1 antibody (A01054-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: rat RH-35 whole cell lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse testis tissue lysates,
Lane 4: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YB1/Ybx1 antigen affinity purified polyclonal antibody (Catalog # A01054-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YB1/Ybx1 at approximately 50 kDa. The expected band size for YB1/Ybx1 is at 36 kDa.
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Flow Cytometry analysis of ANA-1 cells using anti-YB1/Ybx1 antibody (A01054-1).
Overlay histogram showing ANA-1 cells stained with A01054-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-YB1/Ybx1 Antibody (A01054-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-YB1/Ybx1 Antibody Picoband® (A01054-1)
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