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Product Info Summary
| SKU: | A05943 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ZBRK1 ZNF350 Antibody
SKU/Catalog Number
A05943
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZBRK1 ZNF350 Antibody (Catalog # A05943). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZBRK1 ZNF350 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05943)
Host
Rabbit
Contents
ZBRK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZBRK1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human ZBRK1. The immunogen is located within amino acids 410 - 460 of ZBRK1.
Reactive Species
A05943 is reactive to ZNF350 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
60.0 kDa
Background of ZNF350
ZBRK1 is a member of the Kruppel-associated box-zinc finger protein (KRAB-ZFP) protein family of transcriptional repressors. ZBRK1 has been shown to not only be up-regulated in senescent fibroblasts but also interacts with BRCA1, a transcriptional regulator with tumor suppressor activity. ZBRK1 binds to a specific sequence within the GADD45 gene, supporting the assembly of a nuclear complex containing BRCA1 and repressing its transcription. Recent experiments demonstrate that ZBRK1 can homo-oligomerize in vitro and in vivo through a C-terminal transcriptional repression domain (CTRD), and this oligomerization facilitates the ZBRK1-directed transcriptional repression through ZBRK1 response elements. These results suggest that higher order ZBRK1 oligomers may assemble through both protein-DNA and CTRD-dependent protein-protein interactions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05943 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZBRK1 antibody can be used for detection of ZBRK1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZBRK1 in A-20 lysate with ZBRK1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Specific Publications For Anti-ZBRK1 ZNF350 Antibody (A05943)
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