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Product Info Summary
| SKU: | A09282 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-ZBTB1 Antibody
SKU/Catalog Number
A09282
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZBTB1 Antibody (Catalog # A09282). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZBTB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZBTB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09282)
Host
Rabbit
Contents
ZBTB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZBTB1 antibody was raised against a 14 amino acid synthetic peptide near the center of human ZBTB1. The immunogen is located within amino acids 260 - 310 of ZBTB1.
Cross-reactivity
At least two isoforms of ZBTB1 are known to exist. This ZBTB1 antibody will not cross-react with ZBTB2.
Reactive Species
A09282 is reactive to ZBTB1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
82.0 kDa
Background of ZBTB1
ZBTB1 is a zinc finger protein that also contains BTB (BR-C, ttk and bab) domain. While little is known about this protein or the related protein ZBTB2, its expression is known to be down-regulated in human bronchial epithelial cells in response to the presence of Vanadium. Recently, expression of variant ZBTB1 protein has been associated with increased age-related macular degeneration.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09282 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZBTB1 antibody can be used for detection of ZBTB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZBTB1 in HepG2 lysate with ZBTB1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of ZBTB1 in mouse brain tissue with ZBTB1 antibody at 2.5 μg/mL.
Specific Publications For Anti-ZBTB1 Antibody (A09282)
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