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AKT3 antibody (AZD9IL79) performs well in WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of AKT3 using anti-AKT3 antibody (AZD9IL79).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: female zebrafish viscera tissue lysates,
Lane 2: male zebrafish viscera tissue lysates,
Lane 3: whole zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (AZD9IL79) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AKT3 at approximately 56 kDa. The expected band size for AKT3 is at 56 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: female zebrafish viscera tissue lysates,
Lane 2: male zebrafish viscera tissue lysates,
Lane 3: whole zebrafish tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (AZD9IL79) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AKT3 at approximately 56 kDa. The expected band size for AKT3 is at 56 kDa.
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Anti-Zebrafish AKT3 Antibody Picoband®
AKT3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | WB |
Tested Applications
AKT3 antibody was positively detected by WB in:
female zebrafish viscera tissue, male zebrafish viscera tissue, whole zebrafish tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish AKT3 Antibody Picoband®
View all AKT3 Antibodies
View all AKT3 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish AKT3 recombinant protein (Position: M104-L154).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
56 kDa
Background of AKT3
RAC-gamma serine/threonine-protein kinase, also known as protein kinase Akt-3, is an enzyme that in humans is encoded by the AKT3 gene. This gene is mapped to 1q43-q44. The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor(PDGF), insulin, and insulin-like growth factor 1(IGF1). AKT3 plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. This gene is required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands.
Cite This Product
Anti-Zebrafish AKT3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZD9IL79)
Protocols
Western Blot protocol for AKT3 antibody
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Product Promise
We promise Anti-Zebrafish AKT3 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZD9IL79
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026