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- Table of Contents
JUN antibody (AZQ6NZT5) performs well in IF, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (AZQ6NZT5).
c-Jun/JUN was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-c-Jun/JUN Antibody (AZQ6NZT5) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
c-Jun/JUN was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-c-Jun/JUN Antibody (AZQ6NZT5) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Zebrafish c-Jun/JUN Antibody Picoband®
JUN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IF, WB |
Tested Applications
JUN antibody was positively detected by WB in:
zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
JUN antibody was positively detected by IF in:
zebrafish embryo tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunofluorescence
2 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish c-Jun/JUN Antibody Picoband®
View all JUN Antibodies
View all JUN Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish c-Jun/JUN recombinant protein (Position: M1-F308).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of JUN
Enables DNA-binding transcription factor activity, RNA polymerase II-specific. Contributes to DNA-binding transcription factor activity and beta-catenin binding activity. Involved in regulation of transcription by RNA polymerase II. Acts upstream of or within several processes, including canonical Wnt signaling pathway; response to cadmium ion; and response to methylmercury. Part of transcription regulator complex. Is expressed in several structures, including digestive system; hematopoietic system; mesoderm; musculature system; and sensory system. Human ortholog(s) of this gene implicated in pulmonary tuberculosis. Orthologous to human JUN (Jun proto-oncogene, AP-1 transcription factor subunit).
Cite This Product
Anti-Zebrafish c-Jun/JUN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6NZT5)
Protocols
Western Blot protocol for JUN antibody
Immunofluorescence (IF) protocol for JUN antibody
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Product Promise
We promise Anti-Zebrafish c-Jun/JUN Antibody Picoband® performs excellently in relevant applications for IF, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
AZQ6NZT5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026