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Product Info Summary
| SKU: | AZQ6NZT5 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-Zebrafish c-Jun/JUN Antibody Picoband®
SKU/Catalog Number
AZQ6NZT5
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish c-Jun/JUN Antibody Picoband® catalog # AZQ6NZT5. Tested in WB, IF applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish c-Jun/JUN Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6NZT5)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish c-Jun/JUN recombinant protein (Position: M1-F308).
Reactive Species
AZQ6NZT5 is reactive to JUN in Zebrafish
Background of JUN
Enables DNA-binding transcription factor activity, RNA polymerase II-specific. Contributes to DNA-binding transcription factor activity and beta-catenin binding activity. Involved in regulation of transcription by RNA polymerase II. Acts upstream of or within several processes, including canonical Wnt signaling pathway; response to cadmium ion; and response to methylmercury. Part of transcription regulator complex. Is expressed in several structures, including digestive system; hematopoietic system; mesoderm; musculature system; and sensory system. Human ortholog(s) of this gene implicated in pulmonary tuberculosis. Orthologous to human JUN (Jun proto-oncogene, AP-1 transcription factor subunit).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ6NZT5 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Immunofluorescence, 2 μg/ml, Zebrafish
Validation Images & Assay Conditions
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Western blot analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (AZQ6NZT5).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: whole female zebrafish tissue lysates,
Lane 3: whole male zebrafish tissue lysates,
Lane 4: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Jun/JUN antigen affinity purified polyclonal antibody (AZQ6NZT5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for c-Jun/JUN at approximately 39 kDa. The expected band size for c-Jun/JUN is at 33 kDa.
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IF analysis of c-Jun/JUN using anti-c-Jun/JUN antibody (AZQ6NZT5).
c-Jun/JUN was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-c-Jun/JUN Antibody (AZQ6NZT5) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-Zebrafish c-Jun/JUN Antibody Picoband® (AZQ6NZT5)
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