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FGF2 antibody (AZB3DGE3) performs well in WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
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Western blot analysis of FGF2 using anti-FGF2 antibody (AZB3DGE3).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates。After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (AZB3DGE3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17 kDa. The expected band size for FGF2 is at 17 kDa.
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates。After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 antigen affinity purified polyclonal antibody (AZB3DGE3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for FGF2 at approximately 17 kDa. The expected band size for FGF2 is at 17 kDa.
Designing a Western blot for FGF2? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the FGF2 Western Blot GuideBundle Save
Anti-Zebrafish FGF2 Antibody Picoband®
FGF2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | WB |
Tested Applications
FGF2 antibody was positively detected by WB in:
zebrafish head tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish FGF2 Antibody Picoband®
View all FGF2 Antibodies
View all FGF2 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish FGF2 recombinant protein (Position: D14-C154).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of FGF2
Predicted to enable fibroblast growth factor receptor binding activity and growth factor activity. Acts upstream of or within Kupffer's vesicle development. Predicted to be located in extracellular region. Predicted to be active in cytoplasm; extracellular space; and nucleus. Is expressed in brain; eye; forerunner cell group; and kidney. Human ortholog(s) of this gene implicated in several diseases, including Parkinsonism; corneal neovascularization; diabetic neuropathy; gastric ulcer; and impotence. Orthologous to human FGF2 (fibroblast growth factor 2).
Cite This Product
Anti-Zebrafish FGF2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZB3DGE3)
Protocols
Western Blot protocol for FGF2 antibody
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Product Promise
We promise Anti-Zebrafish FGF2 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZB3DGE3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 25, 2026