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Product Info Summary
| SKU: | AZB0UXI3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Zebrafish FOXE3 Antibody
SKU/Catalog Number
AZB0UXI3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish FOXE3 Antibody catalog # AZB0UXI3. Tested in IHC applications. This antibody reacts with Zebrafish.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish FOXE3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZB0UXI3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish FOXE3 recombinant protein (Position: M1-T349).
Reactive Species
AZB0UXI3 is reactive to FOXE3 in Zebrafish
Background of FOXE3
Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within aorta development; lens morphogenesis in camera-type eye; and negative regulation of lens fiber cell differentiation. Predicted to be located in nucleus. Is expressed in several structures, including adenohypophyseal placode; hatching gland; lens; lens placode; and optic primordium. Human ortholog(s) of this gene implicated in anterior segment dysgenesis; anterior segment dysgenesis 2; cataract 34 multiple types; and thoracic aortic aneurysm. Orthologous to human FOXE3 (forkhead box E3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZB0UXI3 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 2-5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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IHC analysis of FOXE3 using anti-FOXE3 antibody (AZB0UXI3).
FOXE3 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXE3 Antibody (AZB0UXI3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of FOXE3 using anti-FOXE3 antibody (AZB0UXI3).
FOXE3 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-FOXE3 Antibody (AZB0UXI3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Zebrafish FOXE3 Antibody (AZB0UXI3)
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