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Product Info Summary
| SKU: | AZQ1LVF8 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish GMDS Antibody Picoband®
SKU/Catalog Number
AZQ1LVF8
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish GMDS Antibody Picoband® catalog # AZQ1LVF8. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish GMDS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ1LVF8)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish GMDS recombinant protein (Position: M1-A370).
Reactive Species
AZQ1LVF8 is reactive to GMDS in Zebrafish
Observed Molecular Weight
40 kDa
Background of GMDS
Enables fucosyltransferase activity. Acts upstream of or within several processes, including central nervous system development; regulation of Notch signaling pathway; and retina layer formation. Is expressed in several structures, including EVL; corneal epithelium; epidermis; neuromast; and periderm. Used to study congenital disorder of glycosylation type IIc. Human ortholog(s) of this gene implicated in open-angle glaucoma. Orthologous to human GMDS (GDP-mannose 4,6-dehydratase).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ1LVF8 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.25-0.5 μg/ml | Zebrafish |
Validation Images & Assay Conditions
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Western blot analysis of GMDS using anti-GMDS antibody (AZQ1LVF8).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: whole female zebrafish tissue lysates,
Lane 3: whole male zebrafish tissue lysates,
Lane 4: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GMDS antigen affinity purified polyclonal antibody (AZQ1LVF8) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GMDS at approximately 40 kDa. The expected band size for GMDS is at 42 kDa.
Specific Publications For Anti-Zebrafish GMDS Antibody Picoband® (AZQ1LVF8)
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