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INTS12 antibody (AZQ6IQU7) performs well in IF, IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of INTS12 using anti-INTS12 antibody (AZQ6IQU7).
INTS12 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-INTS12 Antibody (AZQ6IQU7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
INTS12 was detected in a paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-INTS12 Antibody (AZQ6IQU7) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Zebrafish INTS12 Antibody Picoband®
INTS12 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IF, IHC, WB |
Tested Applications
INTS12 antibody was positively detected by WB in:
zebrafish head tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
INTS12 antibody was positively detected by IHC in:
zebrafish brain tissue, zebrafish spinal cord tissue, zebrafish kidney tissue, zebrafish eye tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
INTS12 antibody was positively detected by IF in:
zebrafish embryo tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
Immunofluorescence
5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish INTS12 Antibody Picoband®
View all INTS12 Antibodies
View all INTS12 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish INTS12 recombinant protein (Position: M1-K394)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
49 kDa
Background of INTS12
Integrator complex subunit 12 (Int12) also known as PHD finger protein 22 (PHF22) is a protein that in humans is encoded by the INTS12 gene. INTS12 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1 (RNU1; MIM 180680) and U2 (RNU2; MIM 180690) (Baillat et al., 2005 [PubMed 16239144]).
Cite This Product
Anti-Zebrafish INTS12 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6IQU7)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Rabbit
63%
Low homology
Sheep
63%
Low homology
Dog
63%
Low homology
Cow
63%
Low homology
Human
62%
Low homology
Monkey
62%
Low homology
Drosophila
25%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for INTS12 antibody
Immunohistochemistry (IHC) protocol for INTS12 antibody
Immunofluorescence (IF) protocol for INTS12 antibody
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Product Promise
We promise Anti-Zebrafish INTS12 Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZQ6IQU7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026