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LHB antibody (AZQ6TCF5) performs well in IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of LHB using anti-LHB antibody (AZQ6TCF5).
LHB was detected in a paraffin-embedded section of zebrafish hypophysis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHB Antibody (AZQ6TCF5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
LHB was detected in a paraffin-embedded section of zebrafish hypophysis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-LHB Antibody (AZQ6TCF5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish LHB Antibody
LHB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC |
Tested Applications
LHB antibody was positively detected by IHC in:
zebrafish hypophysis tissue, zebrafish hypothalamus tissue, zebrafish ovary tissue, zebrafish testis tissue, zebrafish kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish LHB Antibody
View all LHB Antibodies
View all LHB Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish LHB recombinant protein (Position: L24-Y140)
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of LHB
Enables hormone activity. Acts upstream of or within several processes, including female gonad development; oocyte maturation; and ovulation. Predicted to be located in extracellular region. Predicted to be active in cytoplasm and extracellular space. Is expressed in several structures, including endocrine system; female organism; kidney; liver; and male organism. Human ortholog(s) of this gene implicated in hypogonadotropic hypogonadism 23 with or without anosmia. Orthologous to several human genes including LHB (luteinizing hormone subunit beta).
Cite This Product
Anti-Zebrafish LHB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6TCF5)
Protocols
Immunohistochemistry (IHC) protocol for LHB antibody
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AZQ6TCF5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 24, 2026