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Product Info Summary
| SKU: | AZQ8JHZ6 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish p63/TP63 Antibody Picoband®
SKU/Catalog Number
AZQ8JHZ6
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish p63/TP63 Antibody Picoband® catalog # AZQ8JHZ6. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish p63/TP63 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ8JHZ6)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish p63/TP63 recombinant protein (Position: M1-E588).
Reactive Species
AZQ8JHZ6 is reactive to TP63 in Zebrafish
Background of TP63
Enables DNA binding activity. Involved in regulation of ectodermal cell fate specification. Acts upstream of or within several processes, including epidermis morphogenesis; intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; and regulation of programmed necrotic cell death. Located in nucleus. Is expressed in several structures, including brain; digestive system; ectoderm; gonad; and integument. Human ortholog(s) of this gene implicated in several diseases, including ADULT syndrome; EEC syndrome (multiple); ectodermal dysplasia (multiple); orofacial cleft 8; and split hand-foot malformation 4. Orthologous to human TP63 (tumor protein p63).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ8JHZ6 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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Western blot analysis of p63/TP63 using anti-p63/TP63 antibody (AZQ8JHZ6).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p63/TP63 antigen affinity purified polyclonal antibody (AZQ8JHZ6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for p63/TP63 at approximately 63 kDa. The expected band size for p63/TP63 is at 63 kDa.
Specific Publications For Anti-Zebrafish p63/TP63 Antibody Picoband® (AZQ8JHZ6)
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