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PRKCI antibody (AZQ90XF2) performs well in WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of PKC Iota/PRKCI using anti-PKC Iota/PRKCI antibody (AZQ90XF2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC Iota/PRKCI antigen affinity purified polyclonal antibody (AZQ90XF2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PKC Iota/PRKCI at approximately 67 kDa. The expected band size for PKC Iota/PRKCI is at 67 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC Iota/PRKCI antigen affinity purified polyclonal antibody (AZQ90XF2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PKC Iota/PRKCI at approximately 67 kDa. The expected band size for PKC Iota/PRKCI is at 67 kDa.
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Anti-Zebrafish PKC Iota/PRKCI Antibody Picoband®
PRKCI antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | WB |
Tested Applications
PRKCI antibody was positively detected by WB in:
zebrafish embryo tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish PKC Iota/PRKCI Antibody Picoband®
View all PRKCI Antibodies
View all PRKCI Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish PKC Iota/PRKCI recombinant protein (Position: M1-Q547).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of PRKCI
Predicted to enable phospholipid binding activity and protein serine/threonine kinase activity. Acts upstream of with a negative effect on apical protein localization. Acts upstream of or within several processes, including digestive tract development; morphogenesis of an epithelium; and nervous system development. Located in plasma membrane and zonula adherens. Is expressed in several structures, including blastoderm; common cardinal vein; epidermal cell; eye; and posterior lateral line primordium. Orthologous to human PRKCI (protein kinase C iota).
Cite This Product
Anti-Zebrafish PKC Iota/PRKCI Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ90XF2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Zebrafish
100%
High Homology
Cow
90%
High Homology
Sheep
90%
High Homology
Human
89%
Moderate Homology
Rabbit
89%
Moderate Homology
Monkey
89%
Moderate Homology
Rat
89%
Moderate Homology
Mouse
89%
Moderate Homology
Dog
89%
Moderate Homology
Drosophila
68%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for PRKCI antibody
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Product Promise
We promise Anti-Zebrafish PKC Iota/PRKCI Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZQ90XF2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026