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STEAP3 antibody (AZA0A8M1RHF3) performs well in IHC and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of STEAP3 using anti-STEAP3 antibody (AZA0A8M1RHF3).
STEAP3 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STEAP3 Antibody (AZA0A8M1RHF3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
STEAP3 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-STEAP3 Antibody (AZA0A8M1RHF3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Zebrafish STEAP3 Antibody
STEAP3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IHC |
Tested Applications
STEAP3 antibody was positively detected by IHC in:
zebrafish kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish STEAP3 Antibody
View all STEAP3 Antibodies
View all STEAP3 Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived Zebrafish STEAP3 recombinant protein (Position: H327-Y423).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of STEAP3
Predicted to enable cupric reductase (NADH) activity and ferric-chelate reductase (NADPH) activity. Predicted to be involved in copper ion import. Predicted to act upstream of or within iron ion transport. Predicted to be located in endosome membrane. Predicted to be active in endosome and plasma membrane. Is expressed in kidney. Orthologous to human STEAP3 (STEAP3 metalloreductase).
Cite This Product
Anti-Zebrafish STEAP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A8M1RHF3)
Protocols
Immunohistochemistry (IHC) protocol for STEAP3 antibody
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Product Promise
We promise Anti-Zebrafish STEAP3 Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
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AZA0A8M1RHF3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026