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Product Info Summary
| SKU: | AZQ6NYG6 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish TDRD3 Antibody Picoband®
SKU/Catalog Number
AZQ6NYG6
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish TDRD3 Antibody Picoband® catalog # AZQ6NYG6. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish TDRD3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ6NYG6)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish TDRD3 recombinant protein (Position: K216-R242).
Reactive Species
AZQ6NYG6 is reactive to TDRD3 in Zebrafish
Background of TDRD3
Predicted to enable chromatin binding activity; methylated histone binding activity; and transcription coactivator activity. Predicted to act upstream of or within chromatin organization and mRNA processing. Predicted to be located in cytoplasm. Predicted to be part of exon-exon junction complex. Predicted to be active in nucleus. Orthologous to human TDRD3 (tudor domain containing 3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZQ6NYG6 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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Western blot analysis of TDRD3 using anti-TDRD3 antibody (AZQ6NYG6).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDRD3 antigen affinity purified polyclonal antibody (AZQ6NYG6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TDRD3 at approximately 73 kDa. The expected band size for TDRD3 is at 73 kDa.
Specific Publications For Anti-Zebrafish TDRD3 Antibody Picoband® (AZQ6NYG6)
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