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Product Info Summary
| SKU: | AZF1RCY6 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Zebrafish UPF1 Antibody Picoband®
SKU/Catalog Number
AZF1RCY6
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish UPF1 Antibody Picoband® catalog # AZF1RCY6. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish UPF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZF1RCY6)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish UPF1 recombinant protein (Position: F538-D591).
Reactive Species
AZF1RCY6 is reactive to UPF1 in Zebrafish
Background of UPF1
Predicted to enable RNA binding activity and RNA helicase activity. Involved in chordate embryonic development and nuclear-transcribed mRNA catabolic process, nonsense-mediated decay. Acts upstream of or within with a positive effect on positive regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay. Acts upstream of or within T cell differentiation in thymus and regulation of gene expression. Predicted to be located in P-body; nucleus; and perinuclear region of cytoplasm. Predicted to be active in cytoplasm. Orthologous to human UPF1 (UPF1 RNA helicase and ATPase).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
AZF1RCY6 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Zebrafish
Validation Images & Assay Conditions
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Western blot analysis of UPF1 using anti-UPF1 antibody (AZF1RCY6).
Electrophoresis was performed on a 8% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UPF1 antigen affinity purified polyclonal antibody (AZF1RCY6) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for UPF1 at approximately 130 kDa. The expected band size for UPF1 is at 124 kDa.
Specific Publications For Anti-Zebrafish UPF1 Antibody Picoband® (AZF1RCY6)
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