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Product Info Summary
| SKU: | A14319 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-ZF21 ZFYVE21 Antibody
SKU/Catalog Number
A14319
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZF21 ZFYVE21 Antibody (Catalog # A14319). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZF21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZF21 ZFYVE21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14319)
Host
Rabbit
Contents
ZF21 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZF21 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZF21. The immunogen is located within the first 50 amino acids of ZF21.
Cross-reactivity
At least two isoforms of ZF21 are known to exist; this antibody will detect both isoforms.
Reactive Species
A14319 is reactive to ZFYVE21 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
26.5 kDa
Background of ZFYVE21
ZF21 was initially identified as protein that could bind to the cytoplasmic tail of MT1-MMP (Membrane-type 1 matrix metalloproteinase), a potent invasion-promoting protease. ZF21 is a member of a protein family characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain and regulates focal adhesions (FAs) and cell movement. Knockdown of ZF21 expression resulted in a delay of FA disassembly following induction of synchronous disassembly of FAs by nocodazole treatment, suggesting that ZF21 is involved in FA disassembly. ZF21 contains a noncanonical pleckstrin homology domain that is a possible therapeutic target to treat metastatic cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14319 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZF21 antibody can be used for detection of ZF21 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZF21 in 3T3 cell tissue lysate with ZF21 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of ZF21 in human kidney tissue with ZF21 antibody at 20 μg/mL.
Specific Publications For Anti-ZF21 ZFYVE21 Antibody (A14319)
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