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Product Info Summary
| SKU: | A11540 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Chicken |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-ZGPAT Antibody
SKU/Catalog Number
A11540
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZGPAT Antibody (Catalog # A11540). Tested in ELISA, WB, IF applications. This antibody reacts with Human.
Storage & Handling
ZGPAT antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZGPAT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11540)
Host
Chicken
Contents
ZGPAT Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgY
Immunogen
ZGPAT antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ZGPAT. The immunogen is located within the last 50 amino acids of ZGPAT.
Cross-reactivity
Multiple isoforms of ZGPAT are known to exist.
Reactive Species
A11540 is reactive to ZGPAT in Human
Calculated molecular weight
57.4 kDa
Background of ZGPAT
The Zinc finger CCCH-type with G patch domain-containing protein (ZGPAT), is a transcriptional repressor that regulates several cellular pathways including EGFR pathways that are involved in cell proliferation, migration and survival. ZGPAT recruits the Mi-2/NuRD complex and represses the expression of EGFR. ZGPAT is down-regulated in breast carcinomas and its expression is negatively correlated with that of EGFR, suggesting that it may be a potential target for breast cancer therapy. Multiple isoforms of ZGPAT are known to exist, including sZIP, which binds to the Mi-2/NuRD complex but lacks the DNA binding component of full-length ZGPAT, thereby antagonizing transcription repression and growth inhibition by ZGPAT.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11540 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZGPAT antibody can be used for detection of ZGPAT by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZGPAT in SK-N-SH cell lysate with ZGPAT antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunofluorescence of ZGPAT in human brain cells with ZGPAT antibody at 20 μg/mL.
Specific Publications For Anti-ZGPAT Antibody (A11540)
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