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Product Info Summary
| SKU: | A02718-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Zinc Alpha 2 Glycoprotein/AZGP1 Antibody Picoband®
SKU/Catalog Number
A02718-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zinc Alpha 2 Glycoprotein/AZGP1 Antibody Picoband® catalog # A02718-1. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Zinc Alpha 2 Glycoprotein/AZGP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02718-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human Zinc Alpha 2 Glycoprotein/AZGP1 recombinant protein (Position: E22-S298).
Reactive Species
A02718-1 is reactive to AZGP1 in Human, Mouse, Rat
Observed Molecular Weight
41,55 kDa
Calculated molecular weight
34.3 kDa
Background of AZGP1
Predicted to enable RNA nuclease activity and protein transmembrane transporter activity. Involved in cell adhesion and detection of chemical stimulus involved in sensory perception of bitter taste. Located in extracellular space. Implicated in prostate carcinoma. Biomarker of several diseases, including gastrointestinal system cancer (multiple); kidney failure (multiple); liver cirrhosis; lung adenocarcinoma; and obesity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02718-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Western blot analysis of Zinc Alpha 2 Glycoprotein/AZGP1 using anti-Zinc Alpha 2 Glycoprotein/AZGP1 antibody (A02718-1).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HUH-7 whole cell lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Zinc Alpha 2 Glycoprotein/AZGP1 antigen affinity purified polyclonal antibody (A02718-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Zinc Alpha 2 Glycoprotein/AZGP1 at approximately 41,55 kDa. The expected band size for Zinc Alpha 2 Glycoprotein/AZGP1 is at 34 kDa.
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Flow Cytometry analysis of MCF-7 cells using anti-Zinc Alpha 2 Glycoprotein/AZGP1 antibody (A02718-1).
Overlay histogram showing MCF-7 cells stained with A02718-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Zinc Alpha 2 Glycoprotein/AZGP1 Antibody (A02718-1, 1 μg/1x106 cells) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-Zinc Alpha 2 Glycoprotein/AZGP1 Antibody Picoband® (A02718-1)
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