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Product Info Summary
| SKU: | A04761 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-ZIP14 SLC39A14 Antibody
SKU/Catalog Number
A04761
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZIP14 SLC39A14 Antibody (Catalog # A04761). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ZIP14 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZIP14 SLC39A14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04761)
Host
Rabbit
Contents
ZIP14 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZIP14 antibody was raised against a 16 amino acid synthetic peptide near the center of human ZIP14. The immunogen is located within amino acids 230 - 280 of ZIP14.
Cross-reactivity
At least three isoforms of ZIP14 are known to exist; this antibody will detect both isoforms. ZIP14 antibody is predicted to not cross-react with other ZIP family members.
Reactive Species
A04761 is reactive to SLC39A14 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
54.2 kDa
Background of SLC39A14
The zinc transporter ZIP14, also known as SLC39A14, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. The zinc transporter family is divided into four subfamilies (I, II, LIV-1 and gufA). ZIP14 is a glycosylated multipass plasma membrane protein that belongs to the ZIP transporter subfamily LIV-1. ZIP14 has been shown to contribute to the hypozincemia of inflammation and infection and is regulated in the liver by IL-6. In addition to zinc, ZIP14 is also involved in the cellular uptake of non-transferrin-bound iron as well as iron bound to transferrin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04761 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1-4 μg/mL; IHC-P: 1-2 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested.
Validation Images & Assay Conditions
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WB Validation in Human, Mouse and Rat Tissues
Loading: 10 μg of lysate
Antibodies: ZIP14, A04761, 2 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST.
Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of ZIP14 in Human Kidney
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ZIP14 antibody (A04761) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of ZIP14 in Mouse Colon
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-ZIP14 antibody (A04761) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunohistochemistry Validation of ZIP14 in Rat Kideny
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ZIP14 antibody (A04761) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Specific Publications For Anti-ZIP14 SLC39A14 Antibody (A04761)
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