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Product Info Summary
| SKU: | A09389 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ZIP5 SLC39A5 Antibody
SKU/Catalog Number
A09389
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ZIP5 SLC39A5 Antibody (Catalog # A09389). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ZIP5 SLC39A5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09389)
Host
Rabbit
Contents
ZIP5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ZIP5 antibody was raised against a 17 amino acid synthetic peptide near the center of human ZIP5. The immunogen is located within amino acids 330 - 380 of ZIP5.
Reactive Species
A09389 is reactive to SLC39A5 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
56.5 kDa
Background of SLC39A5
The zinc transporter ZIP5, also known as SLC39A5, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP5 is closely related to ZIP4, and is found in many of the same tissues, including intestine, pancreas, liver, and kidney, but unlike ZIP4, ZIP5 localizes to the basolateral membrane in kidney cells and its activity is not downregulated by zinc repletion. Recent evidence suggests that ZIP4 and ZIP5 are reciprocally regulated, with translation of ZIP5 mRNA decreasing and ZIP5 protein being rapidly internalized and degraded in response to zinc deficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09389 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ZIP5 antibody can be used for detection of ZIP5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ZIP5 in human spleen tissue lysate with ZIP5 antibody at 1 μg/mL.
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Immunohistochemistry of ZIP5 in human spleen tissue with ZIP5 antibody at 2.5 μg/mL.
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Immunofluorescence of ZIP5 in human spleen tissue with ZIP5 antibody at 20 μg/mL.
Specific Publications For Anti-ZIP5 SLC39A5 Antibody (A09389)
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