Anti-ZMYM1 Antibody Picoband™

Product Name

Anti-ZMYM1 Antibody Picoband™

View all ZMYM1 Antibodies

SKU/Catalog Number

A18075-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ZMYM1 Antibody Picoband™ catalog # A18075-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ZMYM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18075-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ZMYM1 recombinant protein (Position: R454-E1124).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A18075-1 is reactive to ZMYM1 in Human

Applications

A18075-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

150 kDa

Calculated molecular weight

128.717kDa

Background of ZMYM1

Zinc finger MYM-type containing 1 is a protein that in humans is encoded by the ZMYM1 gene. Predicted to enable protein dimerization activity and zinc ion binding activity. Predicted to be located in nucleus.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ZMYM1 using anti-ZMYM1 antibody (A18075-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human U251 whole cell lysates,
Lane 4: human SiHa whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZMYM1 antigen affinity purified polyclonal antibody (Catalog # A18075-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZMYM1 at approximately 150 kDa. The expected band size for ZMYM1 is at 129 kDa.

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Figure 2. Flow Cytometry analysis of HL-60 cells using anti-ZMYM1 antibody (A18075-1).
Overlay histogram showing HL-60 cells stained with A18075-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZMYM1 Antibody (A18075-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ZMYM1 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ23151; zinc finger, MYM domain containing 1; zinc finger, MYM-type 1 ZMYM1 MYM zinc finger MYM-type containing 1 zinc finger MYM-type protein 1|zinc finger, MYM domain containing 1|zinc finger, MYM-type 1

For more info on ZMYM1, check out the ZMYM1 Infographic

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In this infographic, you will see the following information for ZMYM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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