Anti-ZNF609 Antibody Picoband™

Product Name

Anti-ZNF609 Antibody Picoband™

View all ZNF609 Antibodies

SKU/Catalog Number

A15674-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ZNF609 Antibody Picoband™ catalog # A15674-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ZNF609 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15674-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ZNF609 recombinant protein (Position: R1122-R1411).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A15674-1 is reactive to ZNF609 in Human

Applications

A15674-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

190 kDa

Calculated molecular weight

151.191kDa

Background of ZNF609

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. zinc finger protein 609 (ZNF609) is a 1,411 amino acid member of the Krüppel C2H2-type zinc-finger rotein family. Localized to the nucleus, ZNF609 contains one C2H2-type zinc finger through which it is thought to be involved in DNA-binding and transcriptional regulation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ZNF609 using anti-ZNF609 antibody (A15674-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZNF609 antigen affinity purified polyclonal antibody (Catalog # A15674-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZNF609 at approximately 190 kDa. The expected band size for ZNF609 is at 151 kDa.

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Figure 2. Flow Cytometry analysis of Caco-2 cells using anti-ZNF609 antibody (A15674-1).
Overlay histogram showing Caco-2 cells stained with A15674-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ZNF609 Antibody (A15674-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ZNF609 (Source: Uniprot.org, NCBI)

Alternative Names

KIAA0295; MGC164858; zinc finger protein 609 ZNF609 zinc finger protein 609 zinc finger protein 609

For more info on ZNF609, check out the ZNF609 Infographic

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In this infographic, you will see the following information for ZNF609: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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