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Product Info Summary
| SKU: | M02365 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Zyxin Monoclonal Antibody
SKU/Catalog Number
M02365
BM5649 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Zyxin Monoclonal Antibody catalog # M02365. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Zyxin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02365)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AFOG-26
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Zyxin.
Reactive Species
M02365 is reactive to ZYX in Human, Mouse, Rat
Observed Molecular Weight
78 kDa
Calculated molecular weight
61.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02365 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:500
Positive Control
WB: human MCF-7 whole cell, human PC-3 whole cell, human SIHA whole cell, rat lung tissue, mouse lung tissue
Validation Images & Assay Conditions
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Western blot analysis of Zyxin using anti-Zyxin antibody (M02365).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: rat lung tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Zyxin antigen affinity purified monoclonal antibody (Catalog # M02365) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Zyxin at approximately 78 kDa. The expected band size for Zyxin is at 61 kDa.
Specific Publications For Anti-Zyxin Monoclonal Antibody (M02365)
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6 Customer Q&As for Anti-Zyxin Monoclonal Antibody
Question
Do you have a BSA free version of anti-Zyxin Monoclonal antibody M02365 available?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Zyxin Monoclonal antibody M02365 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-08
Question
Will M02365 anti-Zyxin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
It shows on the product datasheet, M02365 anti-Zyxin Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-23
Question
What sample was tested in WB and IHC applications with M02365?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of Zyxin expression in MCF7 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Would anti-Zyxin Monoclonal antibody M02365 work on horse IP with colon carcinoma?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
Our lab technicians have not tested anti-Zyxin Monoclonal antibody M02365 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Zyxin Monoclonal antibody M02365 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse colon carcinoma in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-23
Question
Does anti-Zyxin Monoclonal antibody M02365 work for IP with colon carcinoma?
Verified Customer
Verified customer
Asked: 2018-12-05
Answer
According to the expression profile of colon carcinoma, ZYX is highly expressed in colon carcinoma. So, it is likely that anti-Zyxin Monoclonal antibody M02365 will work for IP with colon carcinoma.
Boster Scientific Support
Answered: 2018-12-05
Question
We are currently using anti-Zyxin Monoclonal antibody M02365 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?
M. Wu
Verified customer
Asked: 2016-02-01
Answer
The anti-Zyxin Monoclonal antibody (M02365) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-01
