Product Info Summary
SKU: | LS000163 |
---|---|
Size: | 100 µg |
Expression Host: | HEK293T |
Tag: | C-Myc/DDK |
Description: | Transient overexpression lysate of adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 1 |
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Product info
Product Name
AP2B1 (NM_001030006) Human Over-expression Lysate
See all Beta 2 Adaptin products
SKU/Catalog Number
LS000163
Size
100 µg
Description
Transient overexpression lysate of adaptor-related protein complex 2, beta 1 subunit (AP2B1), transcript variant 1
Storage & Handling
Cite This Product
AP2B1 (NM_001030006) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS000163)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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AP2B1 Human Over-expression Lysates NM_001030006.
Western blot validation of overexpression lysate (LS000163) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AP2B1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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AP2B1 Human Over-expression Lysates NM_001282.
Western blot validation of overexpression lysate (LS000163) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AP2B1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Click image to see more details
AP2B1 Human Over-expression Lysates NM_001282.
Western blot validation of overexpression lysate (LS000163) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AP2B1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for AP2B1 (Source: Uniprot.org, NCBI)
Gene Name
AP2B1
Full Name
AP-2 complex subunit beta
Weight
104.553kDa
Superfamily
adaptor complexes large subunit family
Alternative Names
Adapter-related protein complex 2 beta subunit; Adaptor protein complex AP-2 subunit beta; adaptor-related protein complex 2, beta 1 subunit; ADTB2adaptin, beta 2 (beta); AP105B; AP2-BETA; beta-2-adaptin; beta-adaptin; CLAPB1AP-2 complex subunit beta; Clathrin assembly protein complex 2 beta large chain; clathrin-associated/assembly/adaptor protein, large, beta 1; DKFZp781K0743; Plasma membrane adaptor HA2/AP2 adaptin beta subunit AP2B1 ADTB2, AP105B, AP2-BETA, CLAPB1 adaptor related protein complex 2 subunit beta 1 AP-2 complex subunit beta|adapter-related protein complex 2 beta subunit|adapter-related protein complex 2 subunit beta|adaptin, beta 2 (beta)|adaptor protein complex AP-2 subunit beta|adaptor related protein complex 2 beta 1 subunit|adaptor-related protein complex 2 subunit beta|beta-2-adaptin|beta-adaptin|beta2-adaptin|clathrin assembly protein complex 2 beta large chain|clathrin-associated/assembly/adaptor protein, large, beta 1|plasma membrane adaptor HA2/AP2 adaptin beta subunit|testicular tissue protein Li 22
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on AP2B1, check out the AP2B1 Infographic
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In this infographic, you will see the following information for AP2B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For AP2B1 (NM_001030006) Human Over-expression Lysate (LS000163)
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