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Product Info Summary
| SKU: | LS001173 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 1 |
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Product info
Product Name
AP2M1 (NM_004068) Human Over-expression Lysate
SKU/Catalog Number
LS001173
Size
100 µg
Description
Transient overexpression lysate of adaptor-related protein complex 2, mu 1 subunit (AP2M1), transcript variant 1
Storage & Handling
Cite This Product
AP2M1 (NM_004068) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS001173)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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AP2M1 Human Over-expression Lysates NM_001025205.
Western blot validation of overexpression lysate (LS001173) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AP2M1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
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AP2M1 Human Over-expression Lysates NM_004068.
Western blot validation of overexpression lysate (LS001173) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with AP2M1 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for AP2M1 (Source: Uniprot.org, NCBI)
Gene Name
AP2M1
Full Name
AP-2 complex subunit mu
Weight
49.655kDa
Superfamily
adaptor complexes medium subunit family
Alternative Names
Adaptin-mu2; adaptor-related protein complex 2, mu 1 subunit; AP-2 mu chain; Clathrin assembly protein complex 2 medium chain; clathrin coat adaptor protein AP50; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; clathrin-associated/assembly/adaptor protein, medium 1; HA2 50 kDa subunit; KIAA0109; mu subunit; Plasma membrane adaptor AP-2 50 kDa protein; plasma membrane adaptor AP-2 50kDA protein AP2M1 AP50, CLAPM1, MRD60, mu2 adaptor related protein complex 2 subunit mu 1 AP-2 complex subunit mu|AP-2 mu 2 chain|HA2 50 kDA subunit|adaptin-mu2|adaptor protein complex AP-2 subunit mu|adaptor related protein complex 2 mu 1 subunit|adaptor-related protein complex 2 subunit mu|clathrin adaptor complex AP2, mu subunit|clathrin assembly protein complex 2 medium chain|clathrin assembly protein complex 2 mu medium chain|clathrin coat adaptor protein AP50|clathrin coat assembly protein AP50|clathrin coat-associated protein AP50|clathrin-associated/assembly/adaptor protein, medium 1|plasma membrane adaptor AP-2 50 kDa protein|plasma membrane adaptor AP-2 50kDA protein
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on AP2M1, check out the AP2M1 Infographic
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In this infographic, you will see the following information for AP2M1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For AP2M1 (NM_004068) Human Over-expression Lysate (LS001173)
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