Product Info Summary
SKU: | A06179-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-AP-2 complex subunit mu AP2M1 Antibody
SKU/Catalog Number
A06179-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-AP-2 complex subunit mu AP2M1 Antibody catalog # A06179-2. Tested in WB,ICC,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AP-2 complex subunit mu AP2M1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06179-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human StARD13
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06179-2 is reactive to AP2M1 in Human, Mouse, Rat
Applications
A06179-2 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
49.655kDa
Background of AP2M1
GTPase-activating protein for RhoA, and perhaps for Cdc42. May be involved in regulation of cytoskeletal reorganization, cell proliferation and cell motility. Acts a tumor suppressor in hepatocellular carcinoma cells.
Ching Y.-P., J. Biol. Chem. 278:10824-10830(2003).
Nagaraja G.M., Biochem. Biophys. Res. Commun. 313:654-665(2004).
Leung T.H.-Y., Proc. Natl. Acad. Sci. U.S.A. 102:15207-15212(2005).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunocytochemistry staining of AP2M1 using Anti-AP-2 complex subunit mu AP2M1 Antibody (A06179-2).
ICC staining AP2M1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 2. Immunocytochemistry staining of AP2M1 using Anti-AP-2 complex subunit mu AP2M1 Antibody (A06179-2).
ICC staining AP2M1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeProtein Target Info & Infographic
Gene/Protein Information For AP2M1 (Source: Uniprot.org, NCBI)
Gene Name
AP2M1
Full Name
AP-2 complex subunit mu
Weight
49.655kDa
Superfamily
adaptor complexes medium subunit family
Alternative Names
Adaptin-mu2; adaptor-related protein complex 2, mu 1 subunit; AP-2 mu chain; Clathrin assembly protein complex 2 medium chain; clathrin coat adaptor protein AP50; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; clathrin-associated/assembly/adaptor protein, medium 1; HA2 50 kDa subunit; KIAA0109; mu subunit; Plasma membrane adaptor AP-2 50 kDa protein; plasma membrane adaptor AP-2 50kDA protein AP2M1 AP50, CLAPM1, MRD60, mu2 adaptor related protein complex 2 subunit mu 1 AP-2 complex subunit mu|AP-2 mu 2 chain|HA2 50 kDA subunit|adaptin-mu2|adaptor protein complex AP-2 subunit mu|adaptor related protein complex 2 mu 1 subunit|adaptor-related protein complex 2 subunit mu|clathrin adaptor complex AP2, mu subunit|clathrin assembly protein complex 2 medium chain|clathrin assembly protein complex 2 mu medium chain|clathrin coat adaptor protein AP50|clathrin coat assembly protein AP50|clathrin coat-associated protein AP50|clathrin-associated/assembly/adaptor protein, medium 1|plasma membrane adaptor AP-2 50 kDa protein|plasma membrane adaptor AP-2 50kDA protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AP2M1, check out the AP2M1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AP2M1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AP-2 complex subunit mu AP2M1 Antibody (A06179-2)
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No publications found for A06179-2
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