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Product Info Summary
| SKU: | LS079895 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of ATPase, class I, type 8B, member 4 (ATP8B4) |
Product info
Product Name
ATP8B4 (NM_024837) Human Over-expression Lysate
SKU/Catalog Number
LS079895
Size
100 µg
Description
Transient overexpression lysate of ATPase, class I, type 8B, member 4 (ATP8B4)
Storage & Handling
Cite This Product
ATP8B4 (NM_024837) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS079895)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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ATP8B4 Human Over-expression Lysates NM_024837.
Western blot validation of overexpression lysate (LS079895) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with ATP8B4 (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for ATP8B4 (Source: Uniprot.org, NCBI)
Gene Name
ATP8B4
Full Name
Probable phospholipid-transporting ATPase IM
Weight
135.868kDa
Superfamily
cation transport ATPase (P-type) (TC 3.A.3) family
Alternative Names
ATPase class I type 8B member 4; ATPase, class I, type 8B, member 4; ATPIM; EC 3.6.3; EC 3.6.3.1; FLJ25418; KIAA1939; potential phospholipid-transporting ATPase IM; probable phospholipid-transporting ATPase IM ATP8B4 ATPIM ATPase phospholipid transporting 8B4 (putative) probable phospholipid-transporting ATPase IM|ATPase, class I, type 8B, member 4|P4-ATPase flippase complex alpha subunit ATP8B4|potential phospholipid-transporting ATPase IM
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on ATP8B4, check out the ATP8B4 Infographic
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In this infographic, you will see the following information for ATP8B4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For ATP8B4 (NM_024837) Human Over-expression Lysate (LS079895)
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