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- Table of Contents
AK1 · Western blot design guide
AK1 Western Blot Planning Guide
Plan an AK1 Western blot around the catalog-observed 21.6 kDa band, image-backed PB10033 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
AK1 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 21.6 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Lung | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 21.6 kDa |
| ⓘ | Localization | Cytoplasm |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated AK1 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Choroid plexus |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB10033 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected AK1 Western Blot Band Size?
Use the product-observed 21.6 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 21.6 kDa | Matches the authoritative product WB observation. |
| 21.6 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected AK1 appearancePlan around 21.6 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 21.6 kDa; use this as the primary experimental expectation. |
| Calculated mass | 21.6 kDa from UniProt P00568; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB10033. |
| Situation | Likely cause | Next action |
|---|---|---|
| 21.6 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing AK1 band
PB10033 · AK1
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for AK1 Western blot
🧪Use Lung as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Lung (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for AK1
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Lung | Reported tissue cells | High | Protein (HPA) | HPA → |
| Fallopian tube | Reported tissue cells | High | Protein (HPA) | HPA → |
| Choroid plexus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Efferent ducts | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced AK1 Western Blot Tips
Deeper troubleshooting and optimisation questions for AK1, answered from its protein features.
Which band should guide the blot?
Use 21.6 kDa, the observation attached to the authoritative PB10033 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 21.6 kDa expectation.
Which positive control should I start with?
Start with Lung, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for AK1 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB10033 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
AK1 Western Blot Reagents
Human/Mouse/Rat-reactive AK1 Western blot reagents with authoritative product imagery.
Real WB data
Anti-Adenylate Kinase 1/AK1 Antibody Picoband®
Cat # PB10033
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P00568
- Human Protein Atlas — AK1
- PB10033 product record
- PMC3639075 — Monitoring post mortem changes in porcine muscle through 2-D DIGE proteome analysis of Longissimus muscle exudate (Proteome science, 2013)
- PMC13049296 — Investigation of the effects and underlying mechanisms of adenylate kinase 1 on inosine monophosphate deposition in Beijing-You chickens (Poultry science, 2026)