This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
ALDH1A3 · Western blot design guide
ALDH1A3 Western Blot Planning Guide
Plan an ALDH1A3 Western blot around the catalog-observed 56.1 kDa band, image-backed A03030 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
ALDH1A3 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 56.1 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Prostate | |
| Negative control | Appendix |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 56.1 kDa |
| ⓘ | Localization | Cytoplasm |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated ALDH1A3 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Prostate |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A03030 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected ALDH1A3 Western Blot Band Size?
Use the product-observed 56.1 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 56.1 kDa | Matches the authoritative product WB observation. |
| 56.1 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected ALDH1A3 appearancePlan around 56.1 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 56.1 kDa; use this as the primary experimental expectation. |
| Calculated mass | 56.1 kDa from UniProt P47895; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A03030. |
| Situation | Likely cause | Next action |
|---|---|---|
| 56.1 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing ALDH1A3 band
A03030 · ALDH1A3
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for ALDH1A3 Western blot
🧪Use Prostate as the first positive-control candidate and Appendix as the HPA Not detected negative candidate.
Positive control: Prostate (High)
Negative control: Appendix (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for ALDH1A3
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Prostate | Reported tissue cells | High | Protein (HPA) | HPA → |
| Testis | Reported tissue cells | High | Protein (HPA) | HPA → |
| Gallbladder | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Bronchus | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced ALDH1A3 Western Blot Tips
Deeper troubleshooting and optimisation questions for ALDH1A3, answered from its protein features.
Which band should guide the blot?
Use 56.1 kDa, the observation attached to the authoritative A03030 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 56.1 kDa expectation.
Which positive control should I start with?
Start with Prostate, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Appendix as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for ALDH1A3 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A03030 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
ALDH1A3 Western Blot Reagents
Human/Mouse/Rat-reactive ALDH1A3 Western blot reagents with authoritative product imagery.
Real WB data
Anti-ALDH1A3 Antibody Picoband®
Cat # A03030
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P47895
- Human Protein Atlas — ALDH1A3
- A03030 product record
- PMC6030467 — Targeting USP22 Suppresses Tumorigenicity and Enhances Cisplatin Sensitivity Through ALDH1A3 Downregulation in Cancer-Initiating Cells from Lung Adenocarcinoma (Molecular cancer research : MCR, 2018)
- PMC7778538 — ADP-Ribosylation Factor Like GTPase 4C (ARL4C) augments stem-like traits of glioblastoma cells by upregulating ALDH1A3 (Journal of Cancer, 2021)
- PMC8289565 — ALDH1A3 Coordinates Metabolism With Gene Regulation in Pulmonary Arterial Hypertension (Circulation, 2021)