Anti-ALDH1A3 Antibody Picoband™

ALDH1A3 antibody

Boster Bio Anti-ALDH1A3 Antibody Picoband™ catalog # A03030. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A03030
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Product Name

Anti-ALDH1A3 Antibody Picoband™

View all ALDH1A3 Antibodies

SKU/Catalog Number

A03030

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ALDH1A3 Antibody Picoband™ catalog # A03030. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ALDH1A3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03030)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ALDH1A3 recombinant protein (Position: N37-K154). Human ALDH1A3 shares 89% and 87.3% amino acid (aa) sequence identity with mouse and rat ALDH1A3, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A03030 is reactive to ALDH1A3 in Human, Mouse, Rat

Applications

A03030 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

56.108kDa

Background of ALDH1A3

Aldehyde dehydrogenase 1 family, member A3, also known as ALDH1A3 or retinaldehyde dehydrogenase 3 (RALDH3), is an enzyme that in humans is encoded by the ALDH1A3 gene. Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or a cellular retinol-binding protein form. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For ALDH1A3 (Source: Uniprot.org, NCBI)

Gene Name

ALDH1A3

Full Name

Aldehyde dehydrogenase family 1 member A3

Weight

56.108kDa

Superfamily

aldehyde dehydrogenase family

Alternative Names

aldehyde dehydrogenase 1 family, member A3; Aldehyde dehydrogenase 6; aldehyde dehydrogenase family 1 member A3; ALDH1A6; ALDH6acetaldehyde dehydrogenase 6; EC 1.2.1; EC 1.2.1.5; RALDH3; RALDH-3; Retinaldehyde dehydrogenase 3 ALDH1A3 ALDH1A6, ALDH6, MCOP8, RALDH3 aldehyde dehydrogenase 1 family member A3 aldehyde dehydrogenase family 1 member A3|acetaldehyde dehydrogenase 6|aldehyde dehydrogenase 6|retinaldehyde dehydrogenase 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALDH1A3, check out the ALDH1A3 Infographic

ALDH1A3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALDH1A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-ALDH1A3 Antibody Picoband™

Question

We are currently using anti-ALDH1A3 antibody A03030 for mouse tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

The anti-ALDH1A3 antibody (A03030) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-05-01

Question

Is this A03030 anti-ALDH1A3 antibody reactive to the isotypes of ALDH1A3?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

The immunogen of A03030 anti-ALDH1A3 antibody is E.coli-derived human ALDH1A3 recombinant protein (Position: N37-K154). Human ALDH1A3 shares 89% and 87.3% amino acid (aa) sequence identity with mouse and rat ALDH1A3, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-10

Question

I have attached the WB image, lot number and protocol we used for ovarian carcinoma using anti-ALDH1A3 antibody A03030. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-11-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-20

Question

I was wanting to use your anti-ALDH1A3 antibody for Flow Cytometry for rat ovarian carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat ovarian carcinoma identification?

T. Mitchell

Verified customer

Asked: 2019-08-06

Answer

It shows on the product datasheet, A03030 anti-ALDH1A3 antibody has been validated for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat ovarian carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-06

Question

I have a question about product A03030, anti-ALDH1A3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-10-03

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03030 anti-ALDH1A3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-10-03

Question

Is a blocking peptide available for product anti-ALDH1A3 antibody (A03030)?

K. Jha

Verified customer

Asked: 2017-05-12

Answer

We do provide the blocking peptide for product anti-ALDH1A3 antibody (A03030). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-05-12

Question

Will anti-ALDH1A3 antibody A03030 work on zebrafish Flow Cytometry with salivary gland?

A. Jones

Verified customer

Asked: 2016-05-09

Answer

Our lab technicians have not tested anti-ALDH1A3 antibody A03030 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-ALDH1A3 antibody A03030 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish salivary gland in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-05-09

Order DetailsPrice
A03030

100μg

$370
A03030-10ug

10μg sample (liquid)

$99
A03030-Biotin

100 μg Biotin conjugated

$570
A03030-Cy3

100 μg Cy3 conjugated

$570
A03030-Dylight488

100 μg Dylight488 conjugated

$570
A03030-Dylight550

100 μg Dylight550 conjugated

$570
A03030-Dylight594

100 μg Dylight594 conjugated

$570
A03030-FITC

100 μg FITC conjugated

$570
A03030-HRP

100 μg HRP conjugated

$570
A03030-APC

100 μg APC conjugated

$670
A03030-PE

100 μg PE conjugated

$670
A03030-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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Eddy test
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A03030
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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