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- Table of Contents
ATIC · Western blot design guide
ATIC Western Blot Planning Guide
Plan an ATIC Western blot around the catalog-observed 64.6 kDa band, image-backed A05404-2 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
ATIC Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 64.6 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Adrenal gland | |
| Negative control | Seminal vesicle |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 64.6 kDa |
| ⓘ | Localization | Cytoplasm, cytosol |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated ATIC Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Adrenal gland |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A05404-2 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected ATIC Western Blot Band Size?
Use the product-observed 64.6 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 64.6 kDa | Matches the authoritative product WB observation. |
| 64.6 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected ATIC appearancePlan around 64.6 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 64.6 kDa; use this as the primary experimental expectation. |
| Calculated mass | 64.6 kDa from UniProt P31939; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A05404-2. |
| Situation | Likely cause | Next action |
|---|---|---|
| 64.6 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing ATIC band
A05404-2 · ATIC
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for ATIC Western blot
🧪Use Adrenal gland as the first positive-control candidate and Seminal vesicle as the HPA Not detected negative candidate.
Positive control: Adrenal gland (High)
Negative control: Seminal vesicle (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for ATIC
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Adrenal gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Breast | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bone marrow | Reported tissue cells | High | Protein (HPA) | HPA → |
Section 3
Advanced ATIC Western Blot Tips
Deeper troubleshooting and optimisation questions for ATIC, answered from its protein features.
Which band should guide the blot?
Use 64.6 kDa, the observation attached to the authoritative A05404-2 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 64.6 kDa expectation.
Which positive control should I start with?
Start with Adrenal gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Seminal vesicle as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for ATIC Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A05404-2 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
ATIC Western Blot Reagents
Human/Mouse/Rat-reactive ATIC Western blot reagents with authoritative product imagery.
Real WB data
Anti-ATIC Antibody Picoband®
Cat # A05404-2
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P31939
- Human Protein Atlas — ATIC
- A05404-2 product record
- PMC5732395 — Bifunctional enzyme ATIC promotes propagation of hepatocellular carcinoma by regulating AMPK-mTOR-S6 K1 signaling (Cell communication and signaling : CCS, 2017)
- PMC12951375 — ATIC Knockdown Reduces B7-H3 Expression and Oncogenic Signaling in Upper Tract Urothelial Carcinoma Cells (Cancer genomics & proteomics, 2026)
- PMC9905075 — p53 promotes peroxisomal fatty acid β-oxidation to repress purine biosynthesis and mediate tumor suppression (Cell death & disease, 2023)