Anti-ATIC Antibody Picoband™

Product Name

Anti-ATIC Antibody Picoband™

View all ATIC Antibodies

SKU/Catalog Number

A05404-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ATIC Antibody Picoband™ catalog # A05404-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ATIC Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05404-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ATIC recombinant protein (Position: T37-H592).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05404-2 is reactive to ATIC in Human, Mouse, Rat

Applications

A05404-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

64.616kDa

Background of ATIC

Bifunctional purine biosynthesis protein PURH is a protein that in humans is encoded by the ATIC gene. This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ATIC using anti-ATIC antibody (A05404-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human HEK293 whole cell lysates,
Lane 5: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATIC antigen affinity purified polyclonal antibody (Catalog # A05404-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATIC at approximately 65 kDa. The expected band size for ATIC is at 65 kDa.

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Figure 2. Western blot analysis of ATIC using anti-ATIC antibody (A05404-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat stomach tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATIC antigen affinity purified polyclonal antibody (Catalog # A05404-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATIC at approximately 65 kDa. The expected band size for ATIC is at 65 kDa.

Click image to see more details

Figure 3. Flow Cytometry analysis of K562 cells using anti-ATIC antibody (A05404-2).
Overlay histogram showing K562 cells stained with A05404-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATIC Antibody (A05404-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ATIC (Source: Uniprot.org, NCBI)

Alternative Names

5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMPcyclohydrolase; 5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotidetransformylase/inosinicase; AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme; AICAR; AICARFT; AICARFT/IMPCHASE; bifunctional purine biosynthesis protein PURH; IMPCHASE; phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase; PURHFLJ93545 ATIC AICAR, AICARFT, HEL-S-70p, IMPCHASE, PURH 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase bifunctional purine biosynthesis protein ATIC|5-aminoimidazole-4-carboxamide-1-beta-D-ribonucleotide transformylase/inosinicase|AICAR formyltransferase/IMP cyclohydrolase bifunctional enzyme|AICAR transformylase/inosine monophosphate cyclohydrolase|AICARFT/IMPCHASE|bifunctional purine biosynthesis protein PURH|epididymis secretory sperm binding protein Li 70p|phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

For more info on ATIC, check out the ATIC Infographic

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In this infographic, you will see the following information for ATIC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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