ATP1B1 Antibodies

Sodium/potassium-transporting ATPase subunit beta-1 (ATP1B1)

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.

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Gene Information

Human
Gene Name:	ATP1B1
Uniprot:	P05026
Entrez:	481

Family

Belongs to:
X(+)/potassium ATPases subunit beta family

Alternative Names

adenosinetriphosphatase; ATP1B; ATPase, Na+/K+ transporting, beta 1 polypeptide; Beta 1-subunit of Na(+); K(+)-ATPase; MGC1798; Na, K-ATPase beta-1 polypeptide; sodium/potassium-dependent ATPase beta-1 subunit; Sodium/potassium-dependent ATPase subunit beta-1; sodium/potassium-transporting ATPase beta-1 chain; sodium/potassium-transporting ATPase subunit beta-1

Molecular Weight

Mass (kDA):
35.061 kDA

Genomic Context

Human
Location:	1q24.2
Sequence:	1; NC_000001.11 (169106690..169132719)

Tissue Specificity

Found in most tissues.

Subcellular Localizations

Cell membrane; Single-pass type II membrane protein. Cell membrane, sarcolemma. Colocalizes with OBSCN at the intercalated disk and sarcolemma in cardiomyocytes. Localizes in long striations at the level of Z and M lines.

Diseases

• Malignant Neoplasms
• Neoplasms
• Neoplasm Metastasis
• Tissue Adhesions
• Hypertensive Disease
• Hypertrophy
• Malignant Neoplasm Of Breast
• Gastric Adenocarcinoma
• Ovarian Neoplasm
• Adenocarcinoma
• Retinoblastoma
• Mammary Neoplasms
• Essential Hypertension

• Kidney Neoplasm
• Epithelial Ovarian Cancer
• Malignant Paraganglionic Neoplasm
• Prostatic Neoplasms
• Malignant Neoplasm Of Ovary
• Colorectal Cancer
• Cell Invasion

Interacting Proteins

• AGTR1
• ATP1A1
• KIRREL3
• SLC4A1

Pathways

• Transport
• Localization
• Ion Transport
• Cell Proliferation
• Pathogenesis
• Cell Adhesion
• Reverse Transcription
• Cell Cycle
• Cell-cell Adhesion
• Glycosylation
• Ion Homeostasis
• Lymphocyte Activation
• Secretion

• Histone Modification
• Diuresis
• Inflammatory Response
• Blastocyst Formation
• Pronephros Development
• Regulation Of Ion Transport
• Gastric Acid Secretion

PTMs

• Glycosylation
• Phosphorylation

• Glutathionylation
• Deglutathionylation

• Deglycosylation

Research Areas

• Cancer
• Cellular Markers
• Growth and Development
• Hypoxia
• Neuronal Cell Markers

• Neuroscience
• Plasma Membrane Markers
• Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ATP1B1

Best Uses Of The ATP1B1 Marker

If you're looking for an antibody that can detect the ATP1B1 subunit You've come the right site. The Rabbit anti Human ATP1B1 Marker has proven efficient in a variety of colorimetric as well as Enhanced Chemiluminescence (ECL) detection applications. Learn more about this versatile marker below. This antibody is applicable to all scientists and was developed specifically for research in biology.

ATP1B1 is a beta 1 subunit

ATP1B1 is an indicator for the lipid-raft. Its association with NEGR1 is evident in several proteome studies. In the current study GFP-NEGR1 as well as ATP1B1 were both expressed in brain cells of rat transfected by a plasmid expressing the ATP1B1 MYC or a control. The cells were then subjected continuous density gradient centrifugation. Both Ganglioside GM1 and GFP-NEGR1 was found in the raft fraction.

ATP1B1 is a subunit of the sodium-potassium-ATPase. This protein is involved with the transport of potassium-acid throughout the body. It also acts as a marker for sodium and potassium-transporting ATPase. ATP1B1 encodes the b1-subunit. It is also known as 182330. NB3 is the most important subunit of all.

There are two forms of Na,K-ATPase: Atp1b1a1 and atp1b2. Atp1b1 and atp1B2 are expressed at high levels in PV neurons, while Atp1b3 can be found at unobservable levels in this type of cell. Na,K-ATPase is involved in a variety functions within the body, including the regulation of cell proliferation and migration.

Na, K-ATPase b1 and Na K-ATPase Aphetase important for intracellular ion homeostasis and functions specific to tissue. The b-subunit of Na K-ATPase is an adhesion molecule for cells and plays a crucial role in tight junctions as well as polarized epithelial cell.

ATP1B1 and Atp1a3 expression in neuronal tissue differed in brain regions. In the glia, the Atp1b1 gene is expressed at high levels however, Atp1b2 and expression levels are lower. Additionally, Atp1a1 expression is found in neurons with medium-sized somas, but at a low level in interneuron-like small cells.

ATP1B1 has multiple functions. It is an adhesion molecule for cells that plays a role in neural cell migration and neuron-astrocyte adhesion. The malignant growth of gliomas could be caused by the loss of AMOG. The loss of the B1-subunit is associated with an undifferentiated form of phenotype in malignant gliomas.

A study of mRNA levels for ATP1B1 was carried out in the mouse model of Medulloblastoma. The results showed that b1-subunit knockdown cell lines were less prevalent than control cells. In terms of growth, b1 knocked cells were also more tolerant to SAG treatment that Sh-NaKb–cl1.

It is identified by Rabbit anti Human ATP1B1 antibody

Rabbit anti-human ATP1B1 has identified the Boster Bio ATP1B1 marker. This antibody recognizes mRNA found in cells that have ATP1B1 protein. It is also known as ATP1B1 and is used to detect ATP1B1. The mRNA is expressed in numerous tissues and is involved in several cellular processes. Boster Bio ATP1B1 marker is linked to numerous illnesses. It also plays a role in the development of certain types of cancer.

The ATP1B1 antibody recognizes the beta subunit of the ATP synthase, also referred to as F1F0 ATPase. It is present in fungi, plants, and bacteria , and is conserved across all of these species. The beta subunit is responsible regulation of the number of sodium pumps on the plasma membrane. Boster Bio ATP1B1 antibody is able to detect this protein in a variety of biological assays.

The ATP1B1 protein is a member of the Na+/K+ ATPases family. This membrane protein is essential to maintaining the plasma membrane's sodium and potassium electrochemical gradient. It is also vital for the sodium-coupled transportation (transport) of different molecules. This antibody detects the Boster Bio ATP1B1 marker and also its co-immunostained companion, DCFDA.

It is used in Enhanced Chemiluminescence Detection (ECL)

ATP1B1 is a protein that plays a role in the enzyme reaction ATP2A to ATP1. The resulting light is weak, it is visible when an enzyme reacts with a chemically-luminescent substrate. The enhanced chemiluminescence reaction also known as ECL. This method is not suitable for detecting the concentration of proteins in cell membranes.

Comparing to traditional colorimetric methods enhanced chemiluminescence detection offers higher sensitivity and a greater dynamic range. This makes enhanced chemiluminescence an ideal detection method of choice for researchers. In addition, this technology allows researchers to detect high and medium levels of a broad range of biological substances. This is why ATP1B1 is a must in ECL research.

A sample of ATP1B1 is prepared using two different methods. First, the membrane is washed in a buffer containing p-coumaric acid. Next, luminol is added. To a buffer containing 50 units of luminol add 3 ul H2O2. To achieve the desired level luminescence in the ECL solution, the pH ECL solution has to be adjusted.

The enhanced chemiluminescence detection method can be accomplished using x-ray film. This technique is ideal for situations where the levels of protein are high and require high sensitivity. If the samples are too small for Western Blotting or a ECL substrate with a higher specificity may be utilized. Furthermore, enhanced chemiluminescence substrats have higher sensitivity, a longer duration of signal, and greater reproducibility.

It is employed in the colorimetric detection (DAB)

The Boster Bio ATP1B1 marker is highly sensitive and precise in the colorimetric detection. It can be employed for a variety purposes, such as cell proliferation as well as tests for cytotoxicity. Boster's product is extremely reliable and user-friendly for colorimetric detection. This method doesn't require a darkroom.

The Boster Bio ATP1B1 antibody is a monoclonal and polyclonal antibody that is able to bind to ATP1B1 protein in many biological samples. Boster Bio developed the antibodies and they are compatible with human, mouse and rat specimens. The Boster Bio ATP1B1 antibody was developed using mice and rabbits. It is a cellular protein that regulates the number of sodium pumps that are transported to the plasma membrane.