Biotinidase Antibodies

AND BTD ELISA kits, Biotinidase Proteins

Many biological assays use antibodies to detect Biotinidase, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Biotinidase in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Biotinidase antibodies...

We validate the specificity of these antibodies to Biotinidase by testing them on tissues known to express BTD positively and negatively. Browse below to find the BTD antibody that suites your experiment. We have 6 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for Biotinidase? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the BTD IHC Guide

BTD Infographic by Boster Bio

All BTD Products

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Figures

Specificity

Applications

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Anti-PD-L2 PDCD1LG2 Antibody

Western Blot Validation in Human Raji Cell Lysate
Loading: 15 μg of lysates per lane

KO Validation in HeLa Cells
Loading: 15 μg of HeLa WT cell lysates or PD-L2 KO cell lysates

Human, Mouse

ELISA, IF, IHC-P, WB

$481
Cat # A03295
0.1 mg

Anti-PDL2 PDCD1LG2 Monoclonal Antibody [4E10]

Western blot analysis of PD-L2 in overexpressing HEK293 cells PD-L2 antibody at 0

Immunocytochemistry of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 5 μg/mL

Immunofluorescence of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 20 μg/mL

Human

ELISA, Flow Cytometry, IF, IHC-P, ICC, WB

$481
Cat # M03295
0.1 mg

Anti-Biotinidase BTD Antibody

Western blot analysis of extracts of various cell lines, using BTD antibody at 1:1000 dilution

Immunohistochemistry of paraffin-embedded rat brain using BTD antibody at dilution of 1:100

Immunohistochemistry of paraffin-embedded human liver cancer using BTD antibody at dilution of 1:100

Human, Mouse, Rat

IHC, WB

$405
Cat # A03020
100ul

BTD (NM_000060) Human Over-expression Lysate

Human

$628
Cat # LS000686
20 µg, 100 µg

Human Biotinidase/BTD Recombinant Protein

SDS-PAGE

$250
Cat # PROTP43251
Starting from 4ug

Human PD-L2/B7-DC Recombinant Protein

SDS-PAGE

$250
Cat # PROTQ9BQ51
Starting from 10ug

BTD Overview

Facts about Biotinidase.

BTD 3D structure. Source: alphafold

Biotinidase (BTD)

Catalytic release of biotin from biocytin, the product of biotin-dependent carboxylases degradation. .

Gene Information

Human
Gene Name:	BTD
Uniprot:	P43251
Entrez:	686

Family

Belongs to:

carbon-nitrogen hydrolase superfamily

Alternative Names

Biotinase; Biotinidase; BTD; EC 3.5.1; EC 3.5.1.12

Molecular Weight

Mass (kDA):
61.133 kDA

Genomic Context


Human

Location:	3p25.1
Sequence:	3; NC_000003.12 (15601352..15722516)

Subcellular Localizations

Secreted, extracellular space.

Diseases

Biotinidase Deficiency
Bluetongue
Malignant Neoplasms
Cytomegalovirus Infections
Neoplasms
Nervousness
Kyphosis Deformity Of Spine
Biliary Tract Diseases
Pain
Mammary Neoplasms
Multiple Myeloma
Kidney Failure

Rheumatoid Arthritis
Acute Kidney Injury
Fetal Death
Arthritis
Infective Disorder
Tissue Adhesions
Malignant Neoplasm Of Breast

Pathways

Segmentation
Head Development
Secretion
Head Segmentation
Gastrulation
Histone Biotinylation
Limb Development
Cell Differentiation
Chromatin Remodeling
Dna Repair
Transport
Insulin Secretion
Cell Growth

Rna Interference
Cell Adhesion
Ossification
Dosage Compensation
Histone Acetylation
Chromosome Condensation
Cell Proliferation

PTMs

Biotinylation
Cleavage

Oxidation
Reduction

Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BTD

References

PMID: 7509806 by Cole H., et al. Human serum biotinidase. cDNA cloning, sequence, and characterization.

PMID: 9530634 by Knight H.C., et al. Structure of the human biotinidase gene.