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- Table of Contents
BTLA · Western blot design guide
BTLA Western Blot Planning Guide
Plan a BTLA Western blot around the catalog-observed 32.8 kDa band, image-backed A03149 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
BTLA Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 32.8 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 12-15% | |
| Positive control | Lung | |
| Negative control | Adrenal gland |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 32.8 kDa |
| ⓘ | Localization | Cell membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse |
Sample controls for BTLA Western blot
🧪Use Lung as the first positive-control candidate and Adrenal gland as the HPA Not detected negative candidate.
Positive control: Lung (Low)
Negative control: Adrenal gland (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for BTLA
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart on each tissue page — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Lung | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Lymph node | Reported tissue cells | Low | Protein (HPA) | HPA → |
| Tonsil | Reported tissue cells | Low | Protein (HPA) | HPA → |
Section 1
What Is the Expected BTLA Western Blot Band Size?
Use the product-observed 32.8 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 32.8 kDa | Matches the authoritative product WB observation. |
| 32.8 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected BTLA appearancePlan around 32.8 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 32.8 kDa; use this as the primary experimental expectation. |
| Calculated mass | 32.8 kDa from UniProt Q7Z6A9; retain as context. |
| Gel selection | 12-15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A03149. |
| Situation | Likely cause | Next action |
|---|---|---|
| 32.8 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing BTLA band
A03149 · BTLAObserved 32.8 kDa · product-specific WB image
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Section 2
Real Curated BTLA Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Appendix |
| Gel % | 12-15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, standard transfer |
| Membrane | 0.45 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A03149 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 4
Advanced BTLA Western Blot Tips
Deeper troubleshooting and optimisation questions for BTLA, answered from its protein features.
Which band should guide the blot?
Use 32.8 kDa, the observation attached to the authoritative A03149 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 32.8 kDa expectation.
Which positive control should I start with?
Start with Lung, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Adrenal gland as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 12-15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for BTLA Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A03149 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
Compare only the supplied target-verified records: PMC11155569, PMC6704067.
Boster reagents
BTLA Western Blot Reagents
Human/Mouse-reactive BTLA Western blot reagents with authoritative product imagery.
Real WB data
Anti-CD272/BTLA Antibody Picoband®
Cat # A03149
Reactivity: Human, Mouse
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q7Z6A9
- Human Protein Atlas — BTLA
- A03149 product record
- PMC11155569 — BTLA deficiency promotes HSC activation and protects against hepatic ischemia-reperfusion injury.
- PMC6704067 — BTLA suppress acute rejection via regulating TCR downstream signals and cytokines production in kidney transplantation and prolonged allografts survival.