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- Table of Contents
CCL5 · Western blot design guide
CCL5 Western Blot Planning Guide
Plan a CCL5 Western blot around the catalog-observed 10 kDa band, image-backed PB9573 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
CCL5 Western Blot Experimental Design Guide
Expected bands, documented protocol parameters, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 10 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 15% | |
| Positive control | Spleen | |
| Negative control | Bronchus |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 10 kDa |
| ⓘ | Localization | Secreted |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human |
Section 1
Real Curated CCL5 Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Bone marrow |
| Gel % | 15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.2 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | PB9573 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected CCL5 Western Blot Band Size?
Use the product-observed 10 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 10 kDa | Matches the authoritative product WB observation. |
| 10 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected CCL5 appearancePlan around 10 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 10 kDa; use this as the primary experimental expectation. |
| Calculated mass | 10 kDa from UniProt P13501; retain as context. |
| Gel selection | 15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with PB9573. |
| Situation | Likely cause | Next action |
|---|---|---|
| 10 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing CCL5 band
PB9573 · CCL5
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for CCL5 Western blot
🧪Use Spleen as the first positive-control candidate and Bronchus as the HPA Not detected negative candidate.
Positive control: Spleen (High)
Negative control: Bronchus (Not detected)
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for CCL5
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Spleen | Reported tissue cells | High | Protein (HPA) | HPA → |
| Bone marrow | Reported tissue cells | High | Protein (HPA) | HPA → |
| Appendix | Reported tissue cells | Medium | Protein (HPA) | HPA → |
| Endometrium | Reported tissue cells | Medium | Protein (HPA) | HPA → |
Section 3
Advanced CCL5 Western Blot Tips
Deeper troubleshooting and optimisation questions for CCL5, answered from its protein features.
Which band should guide the blot?
Use 10 kDa, the observation attached to the authoritative PB9573 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 10 kDa expectation.
Which positive control should I start with?
Start with Spleen, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use Bronchus as an orthogonal HPA Not detected candidate.
Which gel should I use?
Use 15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for CCL5 Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should PB9573 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
CCL5 Western Blot Reagents
Human-reactive CCL5 Western blot reagents with authoritative product imagery.
Real WB data
Anti-RANTES/CCL5 Antibody Picoband®
Cat # PB9573
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt P13501
- Human Protein Atlas — CCL5
- PB9573 product record
- PMC11633591 — Procaine Regulates the STAT3/CCL5 Axis and Inhibits Microglia M1 Polarization to Alleviate Complete Freund's Adjuvant Rats Pain Behavior (eNeuro, 2024)
- PMC10250326 — Transcriptional control of pancreatic cancer immunosuppression by metabolic enzyme CD73 in a tumor-autonomous and -autocrine manner (Nature communications, 2023)
- PMC7607764 — miR-147a suppresses the metastasis of non-small-cell lung cancer by targeting CCL5 (The Journal of international medical research, 2020)