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- Table of Contents
2 Citations 1 Q&As
1 Citations
Facts about Cyclin-A1.
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Human | |
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Gene Name: | CCNA1 |
Uniprot: | P78396 |
Entrez: | 8900 |
Belongs to: |
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cyclin family |
CCNA1; Cyclin A1; cyclin-A1
Mass (kDA):
52.358 kDA
Human | |
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Location: | 13q13.3 |
Sequence: | 13; NC_000013.11 (36430488..36442882) |
Very high levels in testis and very low levels in brain. Also found in myeloid leukemia cell lines.
Nucleus.
Steven Boster has been a biotechnology pioneer. He created his first product back in 1993 and quickly became known as the man who translates science into the lavatory. He is currently the chief inventor of primary antibodies in China. PicoKine(tm), a proprietary technology delivering high sensitivity ELISA Kits, was also developed by his firm.
There are many choices available when searching for monoclonal/polyclonal antigens antibodies. There are many commercial options, and the quality of the antibody can impact the outcome of an experiment. To ensure the highest quality results, check the antibody quality independently or by looking in the literature. R&D Systems offers quality assurance services to ensure the highest standards. If you prefer to buy directly from the manufacturer, you can be assured of the commercial source control.
The antibody was raised using 8 cell lines to identify alpha (a),-tubulin. It was then confirmed by RTPCR to recognize alpha tubulin proteins. The antibody was generated from goat anti-mouse antigen (#236) and the corresponding secondary antibody, Cat. No. 236-10501. The antibodies were visualized with an enhanced chemiluminescence device after the reaction. The National Institutes of Health (NIH), Image J software, was used to measure the immunoreactive bands.
Selection of primary antibodies is crucial in IHC/ICC experiments. The antibody must be specific for the epitope. All steps must be optimized to maximize specific staining and minimize background signal. Working dilutions for polyclonal antibodies should be determined empirically. Monoclonal and monoclonal antibodies are more sensitive than high-affinity primaries that use the CCNA1 mark.
A polyclonal antibody that has been immobilized in a column is used by an antibody manufacturer to create high-affinity primaries using the CCCA1 mark. The antibody will cross-react and bind to the target protein. The antigen will be recognized by the monoclonal and polyclonal antibodies.
Fluorophore-conjugated antibodies require fewer steps and are less expensive than fluorescent probes. Fluorophore-conjugated antibodies have the added benefit of multiplex compatibility and a reduction in chemical waste. This method can also detect multiple protein species, which is a plus. High-affinity primary antibodies using the CCNA1 marker might be the best choice for many applications.
HPV16 E7 is an antibody with high affinity that binds to HPV-16-transformed cells. This antibody recognises residues 35-56 of the HPV16 protein. This protein is found within the plasma membranes in HPV-transformed tissues. When the antibodies are able to interact with the antigen, the phagocytes become stimulated to take up the pathogen. Research applications will benefit greatly from high-affinity antibodies that use the CCNA1 markers.
The CCNA1 Gene is a gene with unknown function. It is an epitope for CCNA, which is the homologue to CCNA2. It is only expressed in neurons. Recent research has shown it can be useful for the study of brain cancers. The boster bio - Best uses of CCNA1 marker guides contains tips to optimize your experiments.
PMID: 9041194 by Yang R., et al. Characterization of a second human cyclin A that is highly expressed in testis and in several leukemic cell lines.
PMID: 10022926 by Yang R., et al. Functions of cyclin A1 in the cell cycle and its interactions with transcription factor E2F-1 and the Rb family of proteins.
*More publications can be found for each product on its corresponding product page