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CNOT4 Antibodies

AND CNOT4 ELISA kits, CNOT4 Proteins

Many biological assays use antibodies to detect CNOT4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with CNOT4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop CNOT4 antibodies...

We validate the specificity of these antibodies to CNOT4 by testing them on tissues known to express CNOT4 positively and negatively. Browse below to find the CNOT4 antibody that suites your experiment. We have 7 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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CNOT4 Infographic by Boster Bio

CNOT4 protein infographic

All CNOT4 Products

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Anti-CNOT4 Antibody

4 Q&As

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using CNOT4 Antibody

Immunohistochemistry analysis of paraffin-embedded human testis tissue, using CNOT4 Antibody

Western blot analysis of lysates from Jurkat and K562 cells, using CNOT4 Antibody

Human, Mouse, Rat

ELISA, IF, IHC, ICC, WB

$335
Cat # A08405
100ul

CNOT4 (NM_001008225) Human Recombinant Protein

Coomassie blue staining of purified CNOT4 protein

Coomassie blue staining of purified CNOT4 protein

$1249
Cat # PROTO95628
20 µg

Anti-CNOT4 Antibody

Anti-CNOT4 Antibody at 1:8000 dilution + 293 whole cell lysateLysates/proteins at 20 µg per lane

Anti-CNOT4 Antibody at 1:8000 dilution + 293 whole cell lysateLysates/proteins at 20 µg per lane

Human, Mouse

WB

$299
Cat # M08405
80 µl

Anti-CNOT4 Mouse Monoclonal Antibody [Clone ID: OTI3D11]

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CNOT4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CNOT4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Flow cytometric Analysis of Hela cells

Flow cytometric Analysis of Jurkat cells

Human, Mouse, Rat

Flow Cytometry, WB

$523
Cat # M08405-1
100 µl

Anti-CNOT4 Mouse Monoclonal Antibody [Clone ID: OTI1H2]

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (Right lane) cDNA for 48 hrs and lysed

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (Right lane) cDNA for 48 hrs and lysed

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CNOT4 mouse monoclonal antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CNOT4 mouse monoclonal antibody

Human, Mouse, Rat

IHC, WB

$523
Cat # M08405-2
100 µl

CNOT4 (NM_013316) Human Over-expression Lysate

CNOT4 Human Over-expression Lysates NM_001008225

CNOT4 Human Over-expression Lysates NM_001008225

CNOT4 Human Over-expression Lysates NM_001190848

CNOT4 Human Over-expression Lysates NM_013316

Human

$960
Cat # LS004850
20 µg, 100 µg

Anti-CNOT4 Antibody

boster box

Human, Mouse, Rat

ELISA, IF, IHC, ICC, WB

$370
Cat # A08405-1
100 μl/vial

CNOT4 Overview

Facts about CCR4-NOT transcription complex subunit 4.

CNOT4 3D structure

CNOT4 3D structure. Source: alphafold

CCR4-NOT transcription complex subunit 4 (CNOT4)

Has E3 ubiquitin ligase activity, promoting ubiquitination and degradation of target proteins (PubMed:11823428, PubMed:22159038, PubMed:26575292). Involved in activation of this JAK/STAT pathway (PubMed:11823428, PubMed:22159038).

Catalyzes ubiquitination of methylated RBM15 (PubMed:26575292). .

Gene Information

Human
Gene Name:	CNOT4
Uniprot:	O95628
Entrez:	4850

Gene Information

Family

Belongs to:

No superfamily

Family

Alternative Names

CCR4-associated factor 4; CCR4-NOT transcription complex, subunit 4; E3 ubiquitin-protein ligase CNOT4; EC 6.3.2.-; NOT4yeast) homolog

Molecular Weight

Mass (kDA):
63.51 kDA

Molecular Weight

Genomic Context


Human

Location:	7q33
Sequence:	7; NC_000007.14 (135361795..135510127, complement)

Subcellular Localizations

Cytoplasm. Nucleus.

Subcellular Localization

Pathways

Transport
Cell Proliferation
Fertilization
Localization
Embryo Development
Cytokinesis

Pathways

PTMs

Phosphorylation

Bosterbio | ELISA Kits, Antibodies, Antibody Company

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CNOT4

References

PMID: 10637334 by Albert T.K., et al. Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits.

PMID: 11823428 by Albert T.K., et al. Identification of a ubiquitin-protein ligase subunit within the CCR4- NOT transcription repressor complex.