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- Table of Contents
CD177 antigen Antibodies
AND CD177 ELISA kits, CD177 antigen Proteins
Many biological assays use antibodies to detect CD177 antigen, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with CD177 antigen in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop CD177 antigen antibodies...
Designing a Western blot for CD177 antigen? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the CD177 Western Blot GuideDesigning an IHC experiment for CD177 antigen? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the CD177 IHC GuideCD177 Infographic by Boster Bio
All CD177 Products
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CD177 Overview
Facts about CD177 antigen.
CD177 antigen (CD177)
In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407). Additionally, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980).
Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308). However, seems to be dispensable for neutrophil recruitment due to bacterial infection in vivo (PubMed:23461681).
Gene Information
| Human | |
|---|---|
| Gene Name: | CD177 |
| Uniprot: | Q8N6Q3 |
| Entrez: | 57126 |
Family
| Belongs to: |
|---|
| No superfamily |
Alternative Names
CD177 antigenNB1 GP; CD177 molecule; CD177; HNA-2a; HNA2Acell surface receptor; Human neutrophil alloantigen 2a; NB1 GP; NB1PRV-1; polycythemia rubra vera 1; Polycythemia rubra vera protein 1; PRV1; PRV1NB1 glycoprotein
Molecular Weight
Mass (kDA):
46.363 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 19q13.31 |
| Sequence: | 19; NC_000019.10 (43353686..43366063) |
Tissue Specificity
Highly expressed in normal bone marrow and weakly expressed in fetal liver (PubMed:10753836). During neutrophil differentiation, expression begins at the metamyelocyte stage and continues throughout the subsequent stages (at protein level) (PubMed:17244676, PubMed:18462208, PubMed:24926686). Expressed by a subset of mature neutrophils (at protein level) (PubMed:10753836, PubMed:28240246, PubMed:12377969, PubMed:18462208, PubMed:12675722, PubMed:17244676, PubMed:17580308, PubMed:21193407, PubMed:24926686, PubMed:28807980, PubMed:27227454). The percentage of neutrophils expressing CD177 varies across the population (PubMed:17244676, PubMed:27227454). Expressed in granulocytes of patients with polycythemia vera (PV) and with essential thrombocythemia (ET) (PubMed:10753836, PubMed:12377969).
Subcellular Localizations
Cell membrane; Lipid-anchor, GPI-anchor. Membrane raft; Lipid-anchor, GPI-like-anchor. Secreted. Cytoplasmic granule membrane. Cell projection, lamellipodium. Cell surface expression on neutrophils is increased upon TNF-alpha, fMLP or CXCL8/IL8-mediated stimulation (PubMed:17244676, PubMed:17580308). In neutrophils, stored predominantly in secondary and tertiary granules (PubMed:18462208). Can also be shedded from the cell membrane (PubMed:12239154, PubMed:18462208). Localizes to lamellar protrusions in spreading neutrophils (PubMed:28807980).
Diseases
- Polycythemia
- Polycythemia Vera
- Thrombocytosis
- Thrombocythemia, Essential
- Myeloproliferative Disease
- Transfusion Reaction
- Primary Myelofibrosis
- Transfusion-related Acute Lung Injury
- Tissue Adhesions
- Lung Injury
- Acute Lung Injury
- Vasculitis
Interacting Proteins
- KCNIP3
Pathways
- Pathogenesis
- Cell Adhesion
- Neutrophil Activation
- Immune Response
- Cell Activation
- Neutrophil Differentiation
- Phagocytosis
- Cell Differentiation
- Glycosylation
- Localization
- Diapedesis
- Cell Migration
- Fertilization
PTMs
- Glycosylation
- Phosphorylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CD177
bosterbio.com/bosterbio-gene-info-cards/CD177
References
PMID: 10753836 by Temerinac S., et al. Cloning of PRV-1, a novel member of the uPAR receptor superfamily, which is overexpressed in polycythemia rubra vera.
PMID: 11465086 by Kissel K., et al. Molecular basis of the neutrophil glycoprotein NB1 (CD177) involved in the pathogenesis of immunenneutropenia and transfusion reactions.
1 Publications of Boster CD177 antigen Antibodies & ELISA Kits
- PubMed ID: 10.1111/jpn.13664, Chitosan nanoparticles attenuate intestinal damage and inflammatory responses in...
*More publications can be found for each product on its corresponding product page