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- Table of Contents
Facts about Cadherin-3.
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Human | |
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Gene Name: | CDH3 |
Uniprot: | P22223 |
Entrez: | 1001 |
Belongs to: |
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No superfamily |
CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin
Mass (kDA):
91.418 kDA
Human | |
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Location: | 16q22.1 |
Sequence: | 16; NC_000016.10 (68644914..68727573) |
Expressed in some normal epithelial tissues and in some carcinoma cell lines.
Cell membrane; Single-pass type I membrane protein.
Boster Bio's Anti-P-Cadherin-3 CDH3 antibody boster bio product scientists should consider when conducting research in the field biology. Boster antibodies can be used for research and can also be used to aid in species-specific analyses. Researchers who conduct these research studies are entitled to obtain product credits that can then be used for future research. These credits are available to all scientists around the world.
If you're in search of an antibody with high affinity CDH3 marker to conduct your research, Boster Bio has a high-affinity anti-CDH3 antibody. These antibodies have been referenced and validated over 25 years in the past and are highly reliable. Boster Bio offers antibodies to recognize the P cadherin-3 CDH3 protein and is compatible with the Western Blotting, Immunohistochemistry, and ELISA assays.
Antibodies are useful in many applications. Boster Bio's Anti–P–Cadherin3CDH3– one of the examples. The rabbit polyclonal antibody responds to cells from Rat, Mouse, Rat, and Xenopus. Boster Bio's CDH3 Antibody has been confirmed by ELISA and Flow Cytometry methods. It also binds to different proteins like cadherins, integrins and VEGF C.
This antibody recognizes cadherins. They are membrane glycoproteins that create calcium-dependent cell/cell adhesion systems. The CDH3 gene encodes cadherin-3, a transmembrane molecule that consists of five cadherin repeats that are extracellular and an extremely conserved cytoplasmic tail. The CDH3 gene can be found in the long arm of chromosome 16 where it has been associated with breast and prostate cancer. Cervical tumors have also shown abnormal cadherin expression. Genetic mutations in the cadherin-3 gene have been linked to macular degeneration in juveniles and congenital hypotrichosis.
Boster Bio is a manufacturer and distributor of high-specific antibodies. They have invested over two decades in developing their technology and techniques. Their products have been referenced in more than 29,000 publications and are utilized in a variety of research applications. The CDH3 ELISA Kit is compatible with human recombinant CDH3 proteins. This ELISA kit allows researchers to analyze CDH3 in tissue, cell lines, and samples at the level of a picogram.
This antibody reacts with human P cadherin-3 CDH3. It is stored at -20degC over a year, and at 4degC for one month. The reagents contain 5 mg BSA and should be kept at -20C. The Boster Bio CDH3 ELISA kit is able to identify human P cadherin-3. The antibody recognizes human CDH3 protein and its amino acid sequences, 612-626aaqTYDVHLSLSDHGN.
To measure other proteins, an ELISA standard method was used. The ELISA method is used to identify and quantify the protein of interest by using specific antibodies. In certain instances an ELISA reference standard curve can also be created using the staining process of a target protein on parallel slides. This method is particularly helpful for determining molecular weights, or determining the amount of protein found in biological samples. Below are a few examples from proteins that were quantified with ELISA.
An ELISA method detects specific target proteins in Aqueous solutions. These samples may include urine, serum or cells culture media. The primary antibody will bind directly to the protein of interest and has an affinity of high. The antibody, which is usually unlabeled, is called an antibody primary. Detector antibodies are specific in their binding to epitopes. However, some cells express non-specific binding sites, which will interact with the constant region of the detector antibody.
Another method of quantifying protein is sandwich ELISA. This method detects proteins by using multiple antibodies to determine their concentrations. Sandwich ELISA uses multiple antibodies and epitopes to measure the concentration of target proteins. It also measures the specificity and concentration of antibodies. ELISA is the preferred method of measuring other proteins in a quantitative manner. This technology is increasingly being used in biomedical research, as well as in the pharmacy field.
The CDH3 marker was first identified in the soluble fraction of Triton X-100 by immunoprecipitation in zebrafish. It was highly immunoreactive to human PLAC8. It was found to decrease the p-ERK2 level within SC cells, and is associated with reduced ZO-1 immunofluorescence. There are many uncertainties regarding the clinical significance of CDH3 however it is a crucial instrument for detecting a range of diseases.
In the study, researchers discovered 75 candidate proteins taken from the secretomes of GEMM in breast cancer samples. These proteins comprised TOP1 and CDH3. If the GEMM derived secretome could be used to detect human BRCA1 mutations, it will be a huge help in diagnosing breast cancer in these patients. But further studies are necessary before this marker can be utilized in clinical practice. Although the results are encouraging, further research is required before this marker can be utilized in clinical practice as diagnostic tool.
The apical region of CDh2-positive colorectal adenocarcinomas was found to be highly immunoreactive. CDH2 expression was undetectable in HCA-7P8 cells. This suggests that CDh2 subcellular location and ubiquitylation might not be connected. Further research is required to determine the mechanism of CDh2 Ubiquitylation and its connection with CDH3 subcellular localization.
Although its purpose isn't fully understood, researchers have speculated that it could be involved in cell-cell adhesion. Although there is limited empirical evidence, it may affect COAD prognosis. However, the study is the only one to identify CDH3 as a marker of COAD prognosis. This marker could be used to guide the development and implementation of COAD treatment plans.
Although no specific antigen or vaccine has been discovered to target CDH3, this protein is found throughout normal tissues and organs. Studies have proven that plasma levels of CDH3 correlate with the presence of tumors. While this may provide some indication of prognosis but more research is needed to confirm this correlation. Moreover, the findings suggest that CDH3 expression levels could be related to prognosis although further study is necessary.
In addition to its function in the progression of cancer CDH3 might also play a part in colon cancer metastasis. In addition , to its ability to regulate cell adhesion CDH3 is also associated with adhens junctions. In addition, tumor cells with CDH3 expression have lower overall survival rates than those who have normal levels. This finding suggests that CDH3 could be a potential biomarker for colon cancer.
There are currently four large databases that have identified CDH3 genetic changes. We can now look at the methylation status of CDH3 genes as well as the TCGA and the Broad, Grasso and Taylor databases. We can also perform immunoreactivity tests to determine if CDH3 expression is related to the progression of cancer. This is a significant step in the right direction and it will be helpful in early-stage CRC prognosis.
PMID: 2793940 by Shimoyama Y., et al. Molecular cloning of a human Ca2+-dependent cell-cell adhesion molecule homologous to mouse placental cadherin: its low expression in human placental tissues.
PMID: 9815605 by Jarrard D.F., et al. P-cadherin is a basal cell-specific epithelial marker that is not expressed in prostate cancer.
*More publications can be found for each product on its corresponding product page