Deoxyhypusine hydroxylase Antibodies

AND DOHH ELISA kits, Deoxyhypusine hydroxylase Proteins

Many biological assays use antibodies to detect Deoxyhypusine hydroxylase, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Deoxyhypusine hydroxylase in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Deoxyhypusine hydroxylase antibodies...

We validate the specificity of these antibodies to Deoxyhypusine hydroxylase by testing them on tissues known to express DOHH positively and negatively. Browse below to find the DOHH antibody that suites your experiment. We have 1 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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DOHH Infographic by Boster Bio

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Anti-DOHH Rabbit Monoclonal Antibody

Western Blot analysis of HEK-293 whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-DOHH rabbit mAb

Human

IP, WB

$146
Cat # M06330-1
20μL, 40μL, 100μL, 200μL

DOHH Overview

Facts about Deoxyhypusine hydroxylase.

DOHH 3D structure. Source: alphafold

Deoxyhypusine hydroxylase (DOHH)

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)- L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a vital lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the next step of this post-translational modification of the lysine to an unusual amino acid residue termed hypusine (PubMed:16533814, PubMed:16371467, PubMed:19706422).

Hypusination is unique to older eIF-5A variable and is crucial for its function (By similarity). .

Gene Information

Human
Gene Name:	DOHH
Uniprot:	Q9BU89
Entrez:	83475

Family

Belongs to:

deoxyhypusine hydroxylase family

Alternative Names

Deoxyhypusine dioxygenase; deoxyhypusine hydroxylase; deoxyhypusine hydroxylase/monooxygenase; Deoxyhypusine monooxygenase; EC 1.14.99.29; hDOHH; HEAT-like (PBS lyase) repeat containing 1; HEAT-like repeat-containing protein 1; HLRC1; MGC4293

Molecular Weight

Mass (kDA):
32.904 kDA

Genomic Context


Human

Location:	19p13.3
Sequence:	19; NC_000019.10 (3490824..3500674, complement)

Diseases

Malignant Neoplasms
Malaria
Hyperplasia
Malignant Neoplasm Of Prostate
Liver Carcinoma
Communicable Diseases
Glioblastoma
Malignant Paraganglionic Neoplasm
Leishmaniasis, Visceral
Knocked Out
Brain Neoplasms
Diabetes Mellitus

Carcinoma
Immunologic Deficiency Syndromes
Prostatic Neoplasms
Leishmaniasis
Neoplasms
Lymphoma, Follicular

Pathways

Translation
Cell Proliferation
Cell Cycle
Cell Growth
Dna Replication
G1 Phase
Regulation Of Autophagy
Cell Cycle Arrest
Senescence
Drug Resistance

Mrna Processing
Regulation Of Translation
Rna Interference
Autophagy

PTMs

Hydroxylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DOHH

References

PMID: 16533814 by Kim Y.S., et al. Deoxyhypusine hydroxylase is an Fe(II)-dependent, HEAT-repeat enzyme. Identification of amino acid residues critical for Fe(II) binding and catalysis.

PMID: 16371467 by Park J.-H., et al. Molecular cloning, expression, and structural prediction of deoxyhypusine hydroxylase: a HEAT-repeat-containing metalloenzyme.