ECHS1 Antibodies

AND ECHS1 ELISA kits, ECHS1 Proteins

Many biological assays use antibodies to detect ECHS1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with ECHS1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop ECHS1 antibodies...

We validate the specificity of these antibodies to ECHS1 by testing them on tissues known to express ECHS1 positively and negatively. Browse below to find the ECHS1 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing a Western blot for ECHS1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the ECHS1 Western Blot Guide

Designing an IHC experiment for ECHS1? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the ECHS1 IHC Guide

ECHS1 Infographic by Boster Bio

All ECHS1 Products

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Product

Figures

Specificity

Applications

Price

Anti-ECHS1 Antibody Picoband®

Western blot analysis of ECHS1 using anti-ECHS1 antibody (A04508-1)

IHC analysis of ECHS1 using anti-ECHS1 antibody (A04508-1)

+10

Human, Mouse, Rat

ELISA, Flow Cytometry, IF, IHC, ICC, WB

$370
Cat # A04508-1
100 μg/vial

ECHS1 (NM_004092) Human Over-expression Lysate

Human

$628
Cat # LS001892
20 µg, 100 µg

Human ECHS1 Recombinant Protein

SDS-PAGE

$250
Cat # PROTP30084
Starting from 20ug

ECHS1 Overview

Facts about Enoyl-CoA hydratase, mitochondrial.

ECHS1 3D structure. Source: alphafold

Enoyl-CoA hydratase, mitochondrial (ECHS1)

Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA.

It is notable that binds tiglyl-CoA, but hydrates just a small quantity of this substrate. .

Gene Information

Human
Gene Name:	ECHS1
Uniprot:	P30084
Entrez:	1892

Family

Belongs to:

enoyl-CoA hydratase/isomerase family

Alternative Names

EC 4.2.1; enoyl CoA hydratase, short chain, 1, mitochondrial; enoyl Coenzyme A hydratase, short chain, 1, mitochondrial; Enoyl-CoA hydratase 1; enoyl-CoA hydratase, mitochondrial; SCEHEC 4.2.1.17; Short-chain enoyl-CoA hydratase

Molecular Weight

Mass (kDA):
31.387 kDA

Genomic Context


Human

Location:	10q26.3
Sequence:	10; NC_000010.11 (133362480..133373396, complement)

Tissue Specificity

Liver, fibroblast, muscle. Barely detectable in spleen and kidney.

Subcellular Localizations

Mitochondrion matrix.

Diseases

Carcinoma
Malignant Neoplasms
Neoplasms
Fatty Liver
Liver Carcinoma
Diabetes Mellitus
Diabetes Mellitus, Non-insulin-dependent
Liver Neoplasms
Inflammation
Liver Diseases
Nonalcoholic Fatty Liver Disease
Obesity
Hyperplasia

Prion Diseases
Neoplasm Metastasis
Conjunctival Melanoma
Conjunctival Neoplasms
Melanoma
Malignant Neoplasm Of Breast
Sudden Infant Death Syndrome

Interacting Proteins

LRRK2
STAT3
Stat3

Pathways

Fatty Acid Beta-oxidation
Excretion
Transport
Cell Proliferation
Defense Response
Response To Oxidative Stress
Drug Resistance
Regeneration
Flight

Embryo Implantation
Translation
Nucleotide Salvage
Pathogenesis

PTMs

Oxidation

Research Areas

Core ESC-Like Genes
Stem Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ECHS1

References

PMID: 8012501 by Kanazawa M., et al. Molecular cloning and sequence analysis of the cDNA for human mitochondrial short-chain enoyl-CoA hydratase.

PMID: 9073515 by Janssen U., et al. Human mitochondrial enoyl-CoA hydratase gene (ECHS1): structural organization and assignment to chromosome 10q26.2-q26.3.