EIF4G1 Antibodies

Eukaryotic translation initiation factor 4 gamma 1 (EIF4G1)

Part of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. .

Gene Information

Human
Gene Name:	EIF4G1
Uniprot:	Q04637
Entrez:	1981

Family

Belongs to:
eukaryotic initiation factor 4G family

Alternative Names

DKFZp686A1451; EIF4F; eIF-4G 1; eIF4G1; EIF-4G1; eIF-4-gamma 1; EIF4-gamma; EIF4GI; EIF4Gp220; eucaryotic translation initiation factor 4G; eukaryotic translation initiation factor 4 gamma 1; eukaryotic translation initiation factor 4 gamma, 1; P220

Molecular Weight

Mass (kDA):
175.491 kDA

Genomic Context

Human
Location:	3q27.1
Sequence:	3; NC_000003.12 (184314495..184335358)

Diseases

• Neoplasms
• Malignant Neoplasms
• Parkinson Disease
• Foot-and-mouth Disease
• Carcinoma
• Malignant Paraganglionic Neoplasm
• Neoplasm Metastasis
• Malignant Neoplasm Of Breast
• Immunologic Deficiency Syndromes
• Secondary Parkinson Disease
• Cell Invasion
• Picornaviridae Infections

• Mammary Neoplasms
• Malignant Squamous Cell Neoplasm
• Virus Diseases
• Hiv Infections
• Lymphoma
• Infective Disorder

Interacting Proteins

• DDX3X
• EIF3J
• EIF4A1
• EIF4E
• PABPC1

Pathways

• Translation
• Cell Death
• Cell Cycle
• Localization
• Pathogenesis
• Cell Growth
• Proteolysis
• Regulation Of Translation
• Cell Proliferation
• Rna Interference
• Transport
• Gene Silencing
• Induction Of Apoptosis

• Angiogenesis
• Autophagy
• Protein Phosphorylation
• Cell Activation
• Cell Migration
• Programmed Cell Death
• T Cell Activation

PTMs

• Cleavage
• Phosphorylation
• Dephosphorylation

• Farnesylation
• Citrullination
• Methylation

Research Areas

• Cancer
• DNA Repair
• DNA replication, Transcription, Translation and Splicing
• mTOR Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/EIF4G1

Best Uses Of The EIF4G1 Marker

The quality of your primary antibodies can have an impact on the results of research on EIF4G1 synthesis protein synthesis. This article discusses how Boster Bio’s antibody collection can improve your research and the importance of high affinity primary antibodies. You can also learn about typical applications and optimization tips in this guide. The final section of this guide contains a list with questions to ask prior to ordering a primary antibody.

Boster Bio offers high-affinity primary antibodies

Boster offers highly specific and well-cited antibodies against EIF4G1 to aid in protein detection. These antibodies are validated on Western Blotting, Immunohistochemistry, and ELISA. Boster Bio also offers a variety of antisera. The EIF4G1 antibody marker is located within the ER. It is crucial for proper aging as well as cell death.

Typically, a secondary antibodies is created by immunizing a person with an antibody from a different animal species. The characteristics of the immunizing agent determine the specificity and affinity of the secondary antibody. Their specificity and their affinity usually define the quality of primary antibody. A high specificity indicates that a single antibody has high specificity. Good primary antibodies can be used to detect, purify, and measure the antigen of interest.

Optimization tips

Use one of the IHC systems that are optimized for this marker such as Luminex, Boster Bio, or both to maximize your IHC data. Next, select the appropriate secondary antibodies from Boster Bio's extensive selection. Find out the most common questions and best practices regarding negative controls. You can find more information at the Boster Gene Infographics.

Examples of applications

The eIF4G1 marker may be used as a new target for therapy in PCa and other malignancies. It is a key factor in the survival of cancer cells and its overexpression in various malignancies has been linked to oncogenesis. A phage clone representing known proteins of the eIF4G1 gene reacted strongly with serum from PCa patients.

Translation initiation requires eIF4G1. eIF4G1 is less expressed in cancer cells when 53BP1 is metabolically labelled. The protein also inhibits colonies. When the eIF4G1 gene was removed from CRPC cells, it caused a decrease in colony formation. This protein is essential for many biological processes, including the production of prostatic cysts.

When knocked out of cells, eIF4G1 greatly increases the sensitivity of the cells to DNA damage, delay the resolution of DNA damage foci, and reduces overall protein synthesis. While most mRNAs do not use alternate translation, the increased requirement of eIF4G1 facilitates the specialized translation of survival, growth arrest, and DDR mRNAs.

The eIF4G1 protein, which is a component in the eIF4F complex, plays a critical role in the initiation of cap dependent translation. It has three major isoforms, eIF4G1, eIF4GI, and eIF4G3. eIF4G1 has been associated with cap-dependent, while eIF4GI has been associated with IRES independent translation.

The eIF4G1 is a component in the G2/M cycle. It is closely linked to eIF4E and plays a role in the biogenesis 60S ribosomal sulfon. Mutations in eIF4G1 result in decreased levels of 60S ribosomal subunits. A fragment of the C-terminus protein, DAP5, is associated to a pre-60S version. Mass spectrometry analysis confirmed that eIF4G1 is associated with the pre-60S form. Furthermore, eIF4G1 interacts with the Rrp15 and 14 protein.

It has been shown in human cells that it plays a key role in 27S processing (PET maturation) The EIF4G1 marker is useful in detecting these processes in cell cultures. It is useful in vivo experiments to determine if eIF4G1 protein plays a role in cell survival. It could also be used for other types of research in cancer, where tumor growth plays a major role.

Questions to ask about a primary order of antibodies

Boster might be able to answer some questions if you are looking to place an initial order for a primary anti-body. To begin with, you need to know how the antibodies will work. Boster offers a variety secondary antibodies, IHC detection systems, and isotype controls. These are some common questions that you should ask before placing an order.