EN1 Antibodies

AND EN1 ELISA kits, EN1 Proteins

Many biological assays use antibodies to detect EN1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with EN1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop EN1 antibodies...

We validate the specificity of these antibodies to EN1 by testing them on tissues known to express EN1 positively and negatively. Browse below to find the EN1 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Designing an IHC experiment for EN1? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.

Open the EN1 IHC Guide

EN1 Infographic by Boster Bio

All EN1 Products

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Product

Figures

Specificity

Applications

Price

Anti-Engrailed 1 Rabbit Monoclonal Antibody

Western blot analysis of Engrailed 1 expression in 293T cell lysate

Western blot analysis of EN1 using anti-EN1 antibody (M05395)

Human, Mouse, Rat

Flow Cytometry, IP, WB

$370
Cat # M05395
100 μl/vial

Anti-EN1 (Engrailed 1) Antibody (N-term)

A05395 staining EN1 in Human tonsil tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections)

All lanes : Anti-EN1 (Engrailed 1) Antibody (N-term) at 1:2000 dilutionLane 1: U-87 MG whole cell lysateLane 2: U-2OS whole cell lysateLane 3: SH-SY5Y whole cell lysateLane 4: 293T/17 whole cell lysateLane 5: HepG2 whole cell lysateLane 6: Mouse brain lysateLysates/proteins at 20 µg per lane

Fluorescent image of U251 cell stained with EN1 Antibody (N-term)

Human, Mouse

IF, IHC-P, WB

$299
Cat # A05395
50 µl

EN1 (NM_001426) Human Over-expression Lysate

Human

$628
Cat # LS002019
20 µg, 100 µg

EN1 Overview

Facts about Homeobox protein engrailed-1.

EN1 3D structure. Source: alphafold

Homeobox protein engrailed-1 (EN1)

Gene Information

Human
Gene Name:	EN1
Uniprot:	Q05925
Entrez:	2019

Family

Belongs to:

engrailed homeobox family

Alternative Names

engrailed homeobox 1; engrailed homolog 1; Homeobox protein en-1; homeobox protein engrailed-1; hu-En-1

Molecular Weight

Mass (kDA):
40.115 kDA

Genomic Context


Human

Location:	2q14.2
Sequence:	2; NC_000002.12 (118842171..118847648, complement)

Subcellular Localizations

Nucleus.

Diseases

Parkinson Disease
Nervousness
Limb Deformities, Congenital
Neoplasms
Malignant Neoplasms
Congenital Abnormality
Crest Syndrome
Chimera Disorder
Congenital Neurologic Anomalies
Neurodegenerative Disorders
Colorectal Cancer
Nerve Degeneration
Carcinoma

Idiopathic Hypereosinophilic Syndrome
Congenital Absence
Congenital Hand Deformities
Neuroblastoma
Schizophrenia
Syndactyly
Hypoplasia

Pathways

Limb Development
Cell Death
Brain Development
Regionalization
Localization
Cell Proliferation
Neurogenesis
Methylation
Translation
Pathogenesis
Neuron Development
Rna Interference
Midbrain Development

Cerebellum Development
Axon Guidance
Cell Differentiation
Dna Methylation
Hindbrain Development
Programmed Cell Death
Gastrulation

PTMs

Methylation
Phosphorylation
Phosphorothioation

Cleavage
Deacetylation
Acylation

Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/EN1

References

PMID: 1363401 by Logan C., et al. Cloning and sequence comparison of the mouse, human, and chicken engrailed genes reveal potential functional domains and regulatory regions.