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- Table of Contents
Designing a Western blot for Beta-enolase? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ENO3 Western Blot GuideFacts about Beta-enolase.
| Human | |
|---|---|
| Gene Name: | ENO3 |
| Uniprot: | P13929 |
| Entrez: | 2027 |

| Belongs to: |
|---|
| enolase family |

2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; beta-enolase; EC 4.2.1; EC 4.2.1.11; enolase 3 (beta, muscle); Enolase 3; enolase 3, (beta, muscle); GSD13; MSE; Muscle-specific enolase; Skeletal muscle enolase
Mass (kDA):
46.987 kDA

| Human | |
|---|---|
| Location: | 17p13.2 |
| Sequence: | 17; NC_000017.11 (4949182..4957131) |
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Cytoplasm. Localized to the Z line. Some colocalization with CKM at M-band (By similarity).




PMID: 2587223 by Peshavaria M., et al. Structure of human muscle (beta) enolase mRNA and protein deduced from a genomic clone.
PMID: 2336366 by Cali L., et al. Nucleotide sequence of a cDNA encoding the human muscle-specific enolase (MSE).